{"id":298,"date":"2024-11-09T10:39:30","date_gmt":"2024-11-09T10:39:30","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/gene\/?p=298"},"modified":"2024-11-09T10:42:35","modified_gmt":"2024-11-09T10:42:35","slug":"snp%e6%a4%9c%e6%9f%bb%e3%81%ae%e7%8f%be%e7%8a%b6","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/gene\/snp%e6%a4%9c%e6%9f%bb%e3%81%ae%e7%8f%be%e7%8a%b6\/","title":{"rendered":"SNP\u306e\u7279\u5b9a\u65b9\u6cd5"},"content":{"rendered":"\n<p>\u7279\u5b9a\u306eSNP\uff08\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u591a\u578b\uff09\u3092\u691c\u67fb\u3059\u308b\u65b9\u6cd5\u306b\u306f\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u691c\u67fb\u6280\u8853\u304c\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u65b9\u6cd5\u306f\u3001SNP\u306e\u4f4d\u7f6e\u3068\u907a\u4f1d\u7684\u5909\u7570\u3092\u7279\u5b9a\u3059\u308b\u305f\u3081\u306b\u6709\u52b9\u3067\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">1. <strong>PCR\uff08\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff09\u3068RFLP\uff08\u5236\u9650\u9175\u7d20\u65ad\u7247\u9577\u591a\u578b\uff09\u89e3\u6790<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6982\u8981<\/strong>: PCR\u3092\u4f7f\u3063\u3066\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3059\u308bDNA\u9818\u57df\u3092\u5897\u5e45\u3057\u3001\u305d\u306e\u5f8c\u3001\u7279\u5b9a\u306e\u5236\u9650\u9175\u7d20\u3092\u4f7f\u7528\u3057\u3066DNA\u3092\u5207\u65ad\u3057\u307e\u3059\u3002SNP\u304c\u5b58\u5728\u3059\u308b\u5834\u5408\u3001\u5207\u65ad\u30d1\u30bf\u30fc\u30f3\u306b\u9055\u3044\u304c\u751f\u3058\u308b\u305f\u3081\u3001\u907a\u4f1d\u5b50\u591a\u578b\u306e\u6709\u7121\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7279\u5fb4<\/strong>: \u6bd4\u8f03\u7684\u5b89\u4fa1\u3067\u3001\u3088\u304f\u4f7f\u308f\u308c\u308b\u57fa\u672c\u7684\u306a\u65b9\u6cd5\u3067\u3059\u3002\u305f\u3060\u3057\u3001\u51e6\u7406\u304c\u7169\u96d1\u3067\u3001\u7279\u5b9a\u306eSNP\u306b\u7279\u5316\u3057\u3066\u3044\u308b\u305f\u3081\u3001\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u304c\u4f4e\u3044\u3053\u3068\u304c\u30c7\u30e1\u30ea\u30c3\u30c8\u3067\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2. <strong>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09\u3068\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6982\u8981<\/strong>: \u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\uff08\u4f8b: TaqMan\u30d7\u30ed\u30fc\u30d6\uff09\u3092\u4f7f\u7528\u3057\u3066\u3001\u7279\u5b9a\u306eSNP\u3092\u691c\u51fa\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u30d7\u30ed\u30fc\u30d6\u306fSNP\u306e\u5909\u7570\u578b\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3059\u308b\u305f\u3081\u3001\u3069\u306e\u30a2\u30ec\u30eb\u304c\u5b58\u5728\u3059\u308b\u304b\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u5b9a\u91cf\u3067\u304d\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7279\u5fb4<\/strong>: \u9ad8\u611f\u5ea6\u30fb\u9ad8\u7cbe\u5ea6\u3067\u3001\u6bd4\u8f03\u7684\u77ed\u6642\u9593\u3067\u7d50\u679c\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u7279\u306b\u3001\u30bf\u30fc\u30b2\u30c3\u30c8SNP\u304c\u65e2\u77e5\u306e\u5834\u5408\u306b\u52b9\u7387\u7684\u3067\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">3. <strong>DNA\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff08\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff09<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6982\u8981<\/strong>: \u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u6cd5\u3092\u4f7f\u7528\u3057\u3066\u7279\u5b9a\u306eDNA\u9818\u57df\u3092\u8aad\u307f\u53d6\u308a\u3001SNP\u306e\u4f4d\u7f6e\u3068\u5909\u7570\u3092\u76f4\u63a5\u78ba\u8a8d\u3057\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7279\u5fb4<\/strong>: \u9ad8\u7cbe\u5ea6\u3067\u4fe1\u983c\u6027\u306e\u9ad8\u3044\u65b9\u6cd5\u3067\u3059\u304c\u3001\u8cbb\u7528\u304c\u304b\u304b\u308a\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u591a\u3044\u5834\u5408\u306b\u306f\u975e\u52b9\u7387\u3067\u3059\u3002\u5358\u4e00\u307e\u305f\u306f\u5c11\u6570\u306eSNP\u691c\u51fa\u306b\u5411\u3044\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">4. <strong>\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\uff08NGS\uff09<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6982\u8981<\/strong>: NGS\u306f\u3001DNA\u5168\u4f53\u3084\u7279\u5b9a\u306e\u9818\u57df\u3092\u9ad8\u7cbe\u5ea6\u304b\u3064\u9ad8\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u7279\u5b9a\u306eSNP\u3060\u3051\u3067\u306a\u304f\u3001\u4ed6\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3082\u540c\u6642\u306b\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7279\u5fb4<\/strong>: \u591a\u6570\u306eSNP\u3092\u4e00\u5ea6\u306b\u89e3\u6790\u3067\u304d\u308b\u305f\u3081\u3001\u5305\u62ec\u7684\u306a\u907a\u4f1d\u60c5\u5831\u3092\u5f97\u308b\u306e\u306b\u9069\u3057\u3066\u3044\u307e\u3059\u3002\u305f\u3060\u3057\u3001\u30b3\u30b9\u30c8\u304c\u9ad8\u304f\u3001\u7279\u5b9a\u306eSNP\u3060\u3051\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3059\u308b\u5834\u5408\u306b\u306f\u904e\u5270\u306a\u60c5\u5831\u91cf\u3068\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">5. <strong>SNP\u30a2\u30ec\u30a4\uff08DNA\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\uff09<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6982\u8981<\/strong>: \u4e8b\u524d\u306b\u8a2d\u8a08\u3055\u308c\u305f\u30d7\u30ed\u30fc\u30d6\u304c\u30a2\u30ec\u30a4\u4e0a\u306b\u914d\u7f6e\u3055\u308c\u3066\u304a\u308a\u3001\u30b5\u30f3\u30d7\u30ebDNA\u304c\u3053\u308c\u3089\u306e\u30d7\u30ed\u30fc\u30d6\u306b\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u3001\u7279\u5b9a\u306eSNP\u3092\u691c\u51fa\u3057\u307e\u3059\u3002SNP\u306e\u4f4d\u7f6e\u306b\u5fdc\u3058\u3066\u3001\u30a2\u30ec\u30a4\u4e0a\u306e\u4fe1\u53f7\u304c\u5909\u5316\u3057\u3001\u7d50\u679c\u3068\u3057\u3066SNP\u306e\u30bf\u30a4\u30d7\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7279\u5fb4<\/strong>: \u4e00\u5ea6\u306b\u591a\u304f\u306eSNP\u3092\u8fc5\u901f\u306b\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3067\u304d\u308b\u305f\u3081\u3001\u5e45\u5e83\u3044\u907a\u4f1d\u5b50\u60c5\u5831\u3092\u5f97\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u305f\u3060\u3057\u3001\u30a2\u30ec\u30a4\u306b\u306a\u3044SNP\u306f\u691c\u51fa\u3067\u304d\u306a\u3044\u3068\u3044\u3046\u5236\u9650\u304c\u3042\u308a\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">6. <strong>\u30c7\u30b8\u30bf\u30ebPCR\uff08dPCR\uff09<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6982\u8981<\/strong>: dPCR\u306f\u3001DNA\u3092\u591a\u6570\u306e\u5c0f\u3055\u306a\u30d1\u30fc\u30c6\u30a3\u30b7\u30e7\u30f3\u306b\u5206\u3051\u3001\u5404\u30d1\u30fc\u30c6\u30a3\u30b7\u30e7\u30f3\u3067PCR\u3092\u884c\u3044\u3001\u7279\u5b9a\u306eSNP\u3092\u691c\u51fa\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u7279\u5b9a\u306e\u30a2\u30ec\u30eb\u3092\u9ad8\u7cbe\u5ea6\u3067\u5b9a\u91cf\u5316\u3067\u304d\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7279\u5fb4<\/strong>: \u7279\u5b9a\u306eSNP\u306e\u7d76\u5bfe\u5b9a\u91cf\u304c\u53ef\u80fd\u3067\u3001\u4f4e\u983b\u5ea6\u306eSNP\u691c\u51fa\u306b\u3082\u512a\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u4ed6\u306eSNP\u3084\u591a\u578b\u3092\u540c\u6642\u306b\u691c\u51fa\u3059\u308b\u306b\u306f\u9069\u3057\u3066\u3044\u307e\u305b\u3093\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">\u7279\u5b9a\u306eSNP\u691c\u67fb\u3092\u5b9f\u65bd\u3059\u308b\u969b\u306e\u30dd\u30a4\u30f3\u30c8<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u691c\u67fb\u306e\u76ee\u7684\u3068\u8cbb\u7528<\/strong>: \u5fc5\u8981\u3068\u3059\u308b\u60c5\u5831\u91cf\u3084\u7cbe\u5ea6\u306b\u5fdc\u3058\u3066\u3001\u30b3\u30b9\u30c8\u3068\u6642\u9593\u3092\u8003\u616e\u3057\u3001\u6700\u9069\u306a\u65b9\u6cd5\u3092\u9078\u3076\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002<\/li>\n\n\n\n<li><strong>\u691c\u67fb\u6a5f\u95a2\u306e\u5229\u7528<\/strong>: \u907a\u4f1d\u5b50\u691c\u67fb\u306f\u5c02\u9580\u7684\u306a\u8a2d\u5099\u3084\u77e5\u8b58\u3092\u8981\u3059\u308b\u305f\u3081\u3001\u907a\u4f1d\u5b50\u691c\u67fb\u30b5\u30fc\u30d3\u30b9\u3084\u533b\u7642\u6a5f\u95a2\u3067\u306e\u5b9f\u65bd\u304c\u4e00\u822c\u7684\u3067\u3059\u3002<\/li>\n<\/ul>\n<p>\u8a73\u3057\u304f\u306f <a href=\"https:\/\/www.hiro-clinic.or.jp\/gene\/clinic\/\">\u30d2\u30ed\u30af\u30ea\u30cb\u30c3\u30af\u5168\u56fd\u306e\u30af\u30ea\u30cb\u30c3\u30af\u4e00\u89a7<\/a> \u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>","protected":false},"excerpt":{"rendered":"<p>\u7279\u5b9a\u306eSNP\uff08\u5358\u4e00\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u591a\u578b\uff09\u3092\u691c\u67fb\u3059\u308b\u65b9\u6cd5\u306b\u306f\u3001\u4ee5\u4e0b\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u691c\u67fb\u6280\u8853\u304c\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u65b9\u6cd5\u306f\u3001SNP\u306e\u4f4d\u7f6e\u3068\u907a\u4f1d\u7684\u5909\u7570\u3092\u7279\u5b9a\u3059\u308b\u305f\u3081\u306b\u6709\u52b9\u3067\u3059\u3002 1. PCR\uff08\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\uff09\u3068RF [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-298","post","type-post","status-publish","format-standard","hentry","category-latest-technology"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/298","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/comments?post=298"}],"version-history":[{"count":2,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/298\/revisions"}],"predecessor-version":[{"id":300,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/298\/revisions\/300"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/media?parent=298"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/categories?post=298"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/tags?post=298"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}