{"id":301,"date":"2024-11-09T10:45:01","date_gmt":"2024-11-09T10:45:01","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/gene\/?p=301"},"modified":"2024-11-09T10:45:02","modified_gmt":"2024-11-09T10:45:02","slug":"%e3%83%aa%e3%82%a2%e3%83%ab%e3%82%bf%e3%82%a4%e3%83%a0pcr%ef%bc%88qpcr%ef%bc%89%e3%81%a8%e3%82%a2%e3%83%ac%e3%83%ab%e7%89%b9%e7%95%b0%e7%9a%84%e3%83%97%e3%83%ad%e3%83%bc%e3%83%96","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/gene\/%e3%83%aa%e3%82%a2%e3%83%ab%e3%82%bf%e3%82%a4%e3%83%a0pcr%ef%bc%88qpcr%ef%bc%89%e3%81%a8%e3%82%a2%e3%83%ac%e3%83%ab%e7%89%b9%e7%95%b0%e7%9a%84%e3%83%97%e3%83%ad%e3%83%bc%e3%83%96\/","title":{"rendered":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09\u3068\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6"},"content":{"rendered":"\n<p><strong>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09<\/strong>\u3068<strong>\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6<\/strong>\u306f\u3001\u907a\u4f1d\u5b50\u306e\u5b9a\u91cf\u3084\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u5909\u7570\u306e\u691c\u51fa\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u6280\u8853\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u6280\u8853\u306f\u3001\u907a\u4f1d\u5b50\u306e\u89e3\u6790\u3084\u8a3a\u65ad\u3067\u9ad8\u3044\u7cbe\u5ea6\u3068\u7279\u7570\u6027\u3092\u6301\u3061\u3001\u591a\u304f\u306e\u7814\u7a76\u3084\u533b\u7642\u5206\u91ce\u3067\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">1. \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09<\/h3>\n\n\n\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08quantitative PCR\u3001qPCR\uff09\u306f\u3001DNA\u3084RNA\u306e\u7279\u5b9a\u306e\u914d\u5217\u3092\u5897\u5e45\u3057\u3001\u305d\u306e\u5897\u5e45\u904e\u7a0b\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u6280\u8853\u3067\u3059\u3002\u5f93\u6765\u306ePCR\u3068\u7570\u306a\u308a\u3001qPCR\u3067\u306f\u5897\u5e45\u7523\u7269\u306e\u91cf\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u78ba\u8a8d\u3067\u304d\u3001\u76ee\u7684\u306eDNA\u307e\u305f\u306fRNA\u306e\u91cf\u3092\u6b63\u78ba\u306b\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u57fa\u672c\u539f\u7406<\/strong><br>qPCR\u3067\u306f\u3001DNA\u30b5\u30f3\u30d7\u30eb\u3092\u30b5\u30a4\u30af\u30eb\u3054\u3068\u306b\u5897\u5e45\u3057\u3001\u86cd\u5149\u8272\u7d20\u3084\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u3092\u7528\u3044\u3066\u5897\u5e45\u7523\u7269\u306e\u91cf\u3092\u691c\u51fa\u3057\u307e\u3059\u3002DNA\u306e\u5897\u5e45\u91cf\u306b\u6bd4\u4f8b\u3057\u3066\u86cd\u5149\u5f37\u5ea6\u304c\u5897\u52a0\u3059\u308b\u305f\u3081\u3001\u30b5\u30a4\u30af\u30eb\u3054\u3068\u306e\u86cd\u5149\u91cf\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u6e2c\u5b9a\u3057\u3001\u5897\u5e45\u306e\u9032\u884c\u3092\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3057\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7528\u9014<\/strong><\/li>\n\n\n\n<li><strong>\u75c5\u539f\u4f53\u691c\u51fa<\/strong>\uff1a\u611f\u67d3\u75c7\u306e\u8a3a\u65ad\u306b\u5229\u7528\u3055\u308c\u3001\u30a6\u30a4\u30eb\u30b9\u3084\u7d30\u83cc\u306a\u3069\u306e\u75c5\u539f\u4f53\u306e\u5b58\u5728\u3092\u8fc5\u901f\u304b\u3064\u6b63\u78ba\u306b\u691c\u51fa\u3067\u304d\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790<\/strong>\uff1amRNA\u306e\u91cf\u3092\u6e2c\u5b9a\u3057\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u306e\u30ec\u30d9\u30eb\u3092\u5b9a\u91cf\u5316\u3059\u308b\u3053\u3068\u3067\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u304c\u3069\u306e\u7a0b\u5ea6\u767a\u73fe\u3057\u3066\u3044\u308b\u304b\u3092\u8a55\u4fa1\u3057\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u907a\u4f1d\u5b50\u5909\u7570\u306e\u691c\u51fa<\/strong>\uff1a\u304c\u3093\u3084\u907a\u4f1d\u7684\u75be\u60a3\u306b\u95a2\u9023\u3059\u308b\u907a\u4f1d\u5b50\u5909\u7570\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u3082\u7528\u3044\u3089\u308c\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u306e\u7a2e\u985e<\/strong><br>qPCR\u3067\u306f\u3001\u5897\u5e45\u7523\u7269\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u304c\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002\u4ee3\u8868\u7684\u306a\u30d7\u30ed\u30fc\u30d6\u306b\u306f\u300cSYBR Green\u300d\u3068\u300cTaqMan\u30d7\u30ed\u30fc\u30d6\u300d\u304c\u3042\u308a\u307e\u3059\u3002SYBR Green\u306fDNA\u4e8c\u672c\u9396\u306b\u7d50\u5408\u3057\u3066\u86cd\u5149\u3092\u767a\u3057\u3001TaqMan\u30d7\u30ed\u30fc\u30d6\u306f\u7279\u5b9a\u306e\u914d\u5217\u306b\u7279\u7570\u7684\u306b\u7d50\u5408\u3057\u3066\u86cd\u5149\u3092\u767a\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2. \u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6<\/h3>\n\n\n\n<p>\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\uff08allele-specific probe\uff09\u306f\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3084\u591a\u578b\uff08SNP\uff09\u3092\u691c\u51fa\u3059\u308b\u305f\u3081\u306b\u8a2d\u8a08\u3055\u308c\u305f\u30d7\u30ed\u30fc\u30d6\u3067\u3001\u76ee\u7684\u306e\u30a2\u30ec\u30eb\uff08\u907a\u4f1d\u5b50\u306e\u7570\u306a\u308b\u30d0\u30ea\u30a2\u30f3\u30c8\uff09\u306b\u5bfe\u3057\u3066\u9ad8\u3044\u7279\u7570\u6027\u3092\u6301\u3061\u307e\u3059\u3002\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u7570\u306a\u308b\u30a2\u30ec\u30eb\u3092\u6301\u3064\u30b5\u30f3\u30d7\u30eb\u3092\u533a\u5225\u3057\u3001\u907a\u4f1d\u7684\u5909\u7570\u306e\u6709\u7121\u3084\u7a2e\u985e\u3092\u7cbe\u5bc6\u306b\u691c\u51fa\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u57fa\u672c\u539f\u7406<\/strong><br>\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u306a\u308b\u7279\u5b9a\u306e\u30a2\u30ec\u30eb\u306e\u914d\u5217\u306b\u306e\u307f\u7d50\u5408\u3059\u308b\u3088\u3046\u306b\u8a2d\u8a08\u3055\u308c\u307e\u3059\u3002\u30d7\u30ed\u30fc\u30d6\u306b\u86cd\u5149\u8272\u7d20\u3092\u7d44\u307f\u8fbc\u307f\u3001\u30d7\u30ed\u30fc\u30d6\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306b\u7d50\u5408\u3057\u305f\u3068\u304d\u306b\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u3092\u691c\u51fa\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u7279\u5b9a\u306e\u30a2\u30ec\u30eb\u304c\u5b58\u5728\u3059\u308b\u304b\u3069\u3046\u304b\u3092\u5224\u5225\u3067\u304d\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u7528\u9014<\/strong><\/li>\n\n\n\n<li><strong>\u907a\u4f1d\u5b50\u5909\u7570\u306e\u691c\u51fa<\/strong>\uff1a\u304c\u3093\u3084\u907a\u4f1d\u7684\u75be\u60a3\u306b\u95a2\u9023\u3059\u308b\u7279\u5b9a\u306e\u30a2\u30ec\u30eb\u306e\u691c\u51fa\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>\u85ac\u5264\u5fdc\u7b54\u306e\u8a55\u4fa1<\/strong>\uff1a\u85ac\u5264\u306e\u4ee3\u8b1d\u306b\u95a2\u4e0e\u3059\u308b\u907a\u4f1d\u5b50\u306e\u591a\u578b\u3092\u7279\u5b9a\u3057\u3001\u500b\u4eba\u306b\u9069\u3057\u305f\u6cbb\u7642\u65b9\u6cd5\u3092\u9078\u629e\u3059\u308b\u305f\u3081\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>SNP\u30bf\u30a4\u30d4\u30f3\u30b0<\/strong>\uff1a\u907a\u4f1d\u5b50\u591a\u578b\uff08SNP\uff09\u306e\u89e3\u6790\u306b\u7528\u3044\u3089\u308c\u3001\u500b\u3005\u306e\u907a\u4f1d\u7684\u80cc\u666f\u3092\u8abf\u67fb\u3059\u308b\u305f\u3081\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">qPCR\u3068\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u306e\u7d44\u307f\u5408\u308f\u305b<\/h3>\n\n\n\n<p>qPCR\u306b\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3084\u591a\u578b\u3092\u9ad8\u7cbe\u5ea6\u3067\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002\u305f\u3068\u3048\u3070\u3001qPCR\u3067\u5897\u5e45\u3057\u305fDNA\u306b\u5bfe\u3057\u3066\u3001\u5909\u7570\u578b\u3068\u91ce\u751f\u578b\uff08\u901a\u5e38\u306e\u30a2\u30ec\u30eb\uff09\u306b\u305d\u308c\u305e\u308c\u5bfe\u5fdc\u3059\u308b\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u3092\u7528\u3044\u308b\u3068\u3001\u5909\u7570\u306e\u6709\u7121\u3084\u5272\u5408\u3092\u5b9a\u91cf\u7684\u306b\u78ba\u8a8d\u3067\u304d\u308b\u305f\u3081\u3001\u304c\u3093\u3084\u907a\u4f1d\u7684\u75be\u60a3\u306e\u8a3a\u65ad\u3067\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">\u307e\u3068\u3081<\/h3>\n\n\n\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09\u306f\u3001DNA\u3084RNA\u3092\u5897\u5e45\u3057\u306a\u304c\u3089\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u5b9a\u91cf\u7684\u306b\u89e3\u6790\u3059\u308b\u6280\u8853\u3067\u3001\u611f\u67d3\u75c7\u306e\u8a3a\u65ad\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3001\u907a\u4f1d\u5b50\u5909\u7570\u306e\u691c\u51fa\u306a\u3069\u306b\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u306f\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u5909\u7570\u3084\u591a\u578b\u3092\u9ad8\u7cbe\u5ea6\u3067\u8b58\u5225\u3059\u308b\u305f\u3081\u306e\u30d7\u30ed\u30fc\u30d6\u3067\u3042\u308a\u3001qPCR\u3068\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u907a\u4f1d\u7684\u60c5\u5831\u3092\u3088\u308a\u6b63\u78ba\u306b\u89e3\u6790\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u75c5\u6c17\u306e\u8a3a\u65ad\u3084\u500b\u5225\u5316\u533b\u7642\u3001\u7814\u7a76\u306e\u5834\u3067\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u8a73\u3057\u304f\u306f <a href=\"https:\/\/www.hiro-clinic.or.jp\/gene\/clinic\/\">\u30d2\u30ed\u30af\u30ea\u30cb\u30c3\u30af\u5168\u56fd\u306e\u30af\u30ea\u30cb\u30c3\u30af\u4e00\u89a7<\/a> \u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>","protected":false},"excerpt":{"rendered":"<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08qPCR\uff09\u3068\u30a2\u30ec\u30eb\u7279\u7570\u7684\u30d7\u30ed\u30fc\u30d6\u306f\u3001\u907a\u4f1d\u5b50\u306e\u5b9a\u91cf\u3084\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u5909\u7570\u306e\u691c\u51fa\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u6280\u8853\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u6280\u8853\u306f\u3001\u907a\u4f1d\u5b50\u306e\u89e3\u6790\u3084\u8a3a\u65ad\u3067\u9ad8\u3044\u7cbe\u5ea6\u3068\u7279\u7570\u6027\u3092\u6301\u3061\u3001\u591a\u304f\u306e\u7814\u7a76\u3084\u533b\u7642\u5206\u91ce\u3067\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002 1 [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-301","post","type-post","status-publish","format-standard","hentry","category-latest-technology"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/301","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/comments?post=301"}],"version-history":[{"count":1,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/301\/revisions"}],"predecessor-version":[{"id":302,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/301\/revisions\/302"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/media?parent=301"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/categories?post=301"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/tags?post=301"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}