{"id":303,"date":"2024-11-09T10:47:23","date_gmt":"2024-11-09T10:47:23","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/gene\/?p=303"},"modified":"2024-11-09T10:47:24","modified_gmt":"2024-11-09T10:47:24","slug":"qfpcr%e3%81%a8qpcr%e3%81%ae%e9%81%95%e3%81%84","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/gene\/qfpcr%e3%81%a8qpcr%e3%81%ae%e9%81%95%e3%81%84\/","title":{"rendered":"qfPCR\u3068qPCR\u306e\u9055\u3044"},"content":{"rendered":"\n<p><strong>qfPCR<\/strong>\uff08Quantitative Fluorescent PCR\uff09\u3068<strong>qPCR<\/strong>\uff08Quantitative PCR\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff09\u306f\u3001\u3044\u305a\u308c\u3082DNA\u306e\u91cf\u3092\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u6280\u8853\u3067\u3059\u304c\u3001\u4e3b\u306b\u4ee5\u4e0b\u306e\u70b9\u3067\u7570\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\">1. <strong>\u4e3b\u306a\u76ee\u7684\u3068\u7528\u9014\u306e\u9055\u3044<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>qPCR<\/strong><br>qPCR\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067DNA\u3084RNA\u3092\u5897\u5e45\u3057\u3001\u305d\u306e\u5897\u5e45\u904e\u7a0b\u3092\u89b3\u5bdf\u3057\u306a\u304c\u3089\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u306e\u91cf\u3092\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3057\u307e\u3059\u3002qPCR\u306f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3001\u75c5\u539f\u4f53\u691c\u51fa\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u5b9a\u91cf\u306a\u3069\u5e45\u5e83\u3044\u7528\u9014\u306b\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>qfPCR<\/strong><br>qfPCR\u306f\u3001\u67d3\u8272\u4f53\u7570\u5e38\u306e\u691c\u51fa\u3084\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u30b3\u30d4\u30fc\u6570\u306e\u5909\u7570\uff08CNV\uff09\u691c\u51fa\u306b\u7279\u5316\u3057\u3066\u3044\u307e\u3059\u3002\u7279\u306b\u3001\u7279\u5b9a\u306e\u67d3\u8272\u4f53\u306e\u30b3\u30d4\u30fc\u6570\u7570\u5e38\uff08\u4f8b\uff1a\u30c0\u30a6\u30f3\u75c7\u5019\u7fa4\u306a\u3069\u306e\u30c8\u30ea\u30bd\u30df\u30fc\uff09\u306e\u691c\u51fa\u3084\u51fa\u751f\u524d\u8a3a\u65ad\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">2. <strong>\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u4f7f\u7528\u65b9\u6cd5<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>qPCR<\/strong><br>qPCR\u3067\u306f\u3001\u86cd\u5149\u8272\u7d20\uff08\u4f8b\uff1aSYBR Green\uff09\u3084\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\uff08\u4f8b\uff1aTaqMan\u30d7\u30ed\u30fc\u30d6\uff09\u3092\u7528\u3044\u3066\u3001\u5897\u5e45\u7523\u7269\u306e\u751f\u6210\u91cf\u3092\u30b5\u30a4\u30af\u30eb\u3054\u3068\u306b\u30e2\u30cb\u30bf\u30ea\u30f3\u30b0\u3057\u307e\u3059\u3002\u5897\u5e45\u3055\u308c\u305fDNA\u91cf\u306b\u6bd4\u4f8b\u3057\u3066\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u304c\u5f37\u304f\u306a\u308a\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u306e\u89b3\u6e2c\u304c\u53ef\u80fd\u3067\u3059\u3002<\/li>\n\n\n\n<li><strong>qfPCR<\/strong><br>qfPCR\u3067\u306f\u3001\u7279\u5b9a\u306eDNA\u914d\u5217\u306b\u7d50\u5408\u3059\u308b\u86cd\u5149\u30e9\u30d9\u30eb\u4ed8\u304d\u30d7\u30e9\u30a4\u30de\u30fc\u3084\u30d7\u30ed\u30fc\u30d6\u3092\u4f7f\u7528\u3057\u3001\u5897\u5e45\u5f8c\u306b\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u3092\u691c\u51fa\u3057\u307e\u3059\u3002\u5897\u5e45\u904e\u7a0b\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u89b3\u6e2c\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u6700\u7d42\u7684\u306a\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u5f37\u5ea6\u304b\u3089\u5897\u5e45\u7523\u7269\u306e\u91cf\u3092\u5b9a\u91cf\u7684\u306b\u8a55\u4fa1\u3057\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">3. <strong>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u6e2c\u5b9a\u306e\u6709\u7121<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>qPCR<\/strong><br>qPCR\u306f\u3001\u5897\u5e45\u30b5\u30a4\u30af\u30eb\u3054\u3068\u306b\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u304c\u5897\u52a0\u3059\u308b\u305f\u3081\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u306e\u6e2c\u5b9a\u304c\u53ef\u80fd\u3067\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u91cf\u3092\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u306b\u8ffd\u8de1\u3067\u304d\u3001\u5897\u5e45\u52b9\u7387\u3082\u8a55\u4fa1\u3067\u304d\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>qfPCR<\/strong><br>qfPCR\u306f\u3001\u5897\u5e45\u30b5\u30a4\u30af\u30eb\u3054\u3068\u306b\u89b3\u6e2c\u3059\u308b\u306e\u3067\u306f\u306a\u304f\u3001\u5897\u5e45\u5f8c\u306e\u86cd\u5149\u5f37\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u3001qPCR\u306e\u3088\u3046\u306a\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u89b3\u6e2c\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u6700\u7d42\u7684\u306a\u5b9a\u91cf\u8a55\u4fa1\u306b\u91cd\u70b9\u3092\u7f6e\u3044\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">4. <strong>\u7528\u9014\u306b\u5fdc\u3058\u305f\u8a2d\u8a08<\/strong><\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>qPCR<\/strong>\u306f\u3001\u4e00\u822c\u7684\u306a\u5b9a\u91cfPCR\u3067\u5e45\u5e83\u3044\u7528\u9014\u306b\u5bfe\u5fdc\u3057\u3066\u304a\u308a\u3001\u7279\u5b9a\u306e\u907a\u4f1d\u5b50\u3084\u30a6\u30a4\u30eb\u30b9\u91cf\u3092\u9ad8\u7cbe\u5ea6\u306b\u5b9a\u91cf\u3059\u308b\u76ee\u7684\u3067\u4f7f\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>qfPCR<\/strong>\u306f\u3001\u67d3\u8272\u4f53\u6570\u3084\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u5909\u7570\u306e\u691c\u51fa\u306b\u7279\u5316\u3057\u3066\u304a\u308a\u3001\u8fc5\u901f\u306a\u8a3a\u65ad\u7d50\u679c\u304c\u6c42\u3081\u3089\u308c\u308b\u51fa\u751f\u524d\u8a3a\u65ad\u3084\u67d3\u8272\u4f53\u7570\u5e38\u691c\u51fa\u3067\u7279\u306b\u6709\u52b9\u3067\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\">\u307e\u3068\u3081<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>qPCR<\/strong>\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0\u3067\u907a\u4f1d\u5b50\u306e\u91cf\u3092\u8ffd\u8de1\u30fb\u5b9a\u91cf\u3067\u304d\u3001\u5e45\u5e83\u3044\u907a\u4f1d\u5b50\u89e3\u6790\u3084\u767a\u73fe\u89e3\u6790\u306b\u5229\u7528\u3055\u308c\u307e\u3059\u3002<\/li>\n\n\n\n<li><strong>qfPCR<\/strong>\u306f\u3001\u67d3\u8272\u4f53\u6570\u7570\u5e38\u3084\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u5909\u7570\u3092\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u3067\u6700\u7d42\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u624b\u6cd5\u3067\u3001\u67d3\u8272\u4f53\u7570\u5e38\u306e\u8a3a\u65ad\u3084\u51fa\u751f\u524d\u8a3a\u65ad\u306b\u9069\u3057\u3066\u3044\u307e\u3059\u3002<\/li>\n<\/ul>\n\n\n\n<p>\u3053\u306e\u3088\u3046\u306b\u3001qPCR\u3068qfPCR\u306f\u4f7f\u7528\u3059\u308b\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u65b9\u6cd5\u3084\u4e3b\u306a\u7528\u9014\u306b\u9055\u3044\u304c\u3042\u308a\u3001\u305d\u308c\u305e\u308c\u306e\u76ee\u7684\u306b\u5fdc\u3058\u3066\u6700\u9069\u306a\u6280\u8853\u304c\u9078\u3070\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u8a73\u3057\u304f\u306f <a href=\"https:\/\/www.hiro-clinic.or.jp\/gene\/clinic\/\">\u30d2\u30ed\u30af\u30ea\u30cb\u30c3\u30af\u5168\u56fd\u306e\u30af\u30ea\u30cb\u30c3\u30af\u4e00\u89a7<\/a> \u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>","protected":false},"excerpt":{"rendered":"<p>qfPCR\uff08Quantitative Fluorescent PCR\uff09\u3068qPCR\uff08Quantitative PCR\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff09\u306f\u3001\u3044\u305a\u308c\u3082DNA\u306e\u91cf\u3092\u5b9a\u91cf\u7684\u306b\u6e2c\u5b9a\u3059\u308b\u6280\u8853\u3067\u3059\u304c\u3001\u4e3b\u306b\u4ee5\u4e0b\u306e\u70b9\u3067\u7570\u306a\u308a\u307e\u3059\u3002 1. [&hellip;]<\/p>\n","protected":false},"author":9,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-303","post","type-post","status-publish","format-standard","hentry","category-latest-technology"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/303","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/users\/9"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/comments?post=303"}],"version-history":[{"count":1,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/303\/revisions"}],"predecessor-version":[{"id":304,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/posts\/303\/revisions\/304"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/media?parent=303"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/categories?post=303"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/gene\/wp-json\/wp\/v2\/tags?post=303"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}