{"id":2584,"date":"2024-09-01T09:42:12","date_gmt":"2024-09-01T00:42:12","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/nippt\/%e6%ac%a1%e4%b8%96%e4%bb%a3%e3%82%b7%e3%83%bc%e3%82%af%e3%82%a8%e3%83%b3%e3%82%b5%e3%83%bc%e3%81%8c%e5%a4%9a%e6%95%b0%e3%81%aepcr%e3%81%a8%e7%a7%b0%e3%81%95%e3%82%8c%e3%82%8b%e3%82%8f%e3%81%91\/"},"modified":"2024-09-12T05:40:12","modified_gmt":"2024-09-12T05:40:12","slug":"%e6%ac%a1%e4%b8%96%e4%bb%a3%e3%82%b7%e3%83%bc%e3%82%af%e3%82%a8%e3%83%b3%e3%82%b5%e3%83%bc%e3%81%8c%e5%a4%9a%e6%95%b0%e3%81%aepcr%e3%81%a8%e7%a7%b0%e3%81%95%e3%82%8c%e3%82%8b%e3%82%8f%e3%81%91","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/nippt\/%e6%ac%a1%e4%b8%96%e4%bb%a3%e3%82%b7%e3%83%bc%e3%82%af%e3%82%a8%e3%83%b3%e3%82%b5%e3%83%bc%e3%81%8c%e5%a4%9a%e6%95%b0%e3%81%aepcr%e3%81%a8%e7%a7%b0%e3%81%95%e3%82%8c%e3%82%8b%e3%82%8f%e3%81%91\/?lang=en","title":{"rendered":"Why the next generation of sequencers is called PCR for many"},"content":{"rendered":"\n

Next-generation sequencing (NGS) and polymerase chain reaction (PCR)<\/strong> are both techniques used to identify DNA sequences, but there are significant there are differences. The basic differences between these two technologies are explained in simple terms.<\/p>\n\n\n\n\n\n

PCR (polymerase chain reaction)<\/h3>\n\n\n\n

PCR is a method of selectively replicating specific DNA regions in large numbers<\/strong>.Short DNA primers<\/strong> are used to identify the DNA region of interest and the DNA polymerase enzyme is used to<\/strong>replicate the region thousands or tens of thousands of times.This allows even very small amounts of DNA samples to produce analyzable quantities of DNA.<\/strong>PCR is relatively simple and fast and is suitable for detecting the presence of specific genes.<\/p>\n\n\n\n

Next generation sequencers (NGS)<\/h3>\n\n\n\n

Next-generation sequencing<\/strong> is a technique for sequencing many DNA fragments simultaneously at high speed, in<\/strong> contrast to PCR, which amplifies a single DNA region. NGS begins by DNA randomly fragmented into many small pieces <\/strong>and a short DNA sequence called an adaptor is added to<\/strong> each fragment. These fragments are fixed to a surface called a flow cell<\/strong>, where each fragment<\/strong> is sequenced simultaneously and in parallel<\/strong>. In each cycle, nucleotides with fluorescent markers<\/strong> are added and their fluorescence is read by a laser to<\/strong> determine the DNA sequence.<\/p>\n\n\n\n

PCR and NGS.<\/h3>\n\n\n\n

PCR technology is sometimes used in the NGS process; it is common for PCR to be used to amplify specific DNA fragments<\/strong> during the sample preparation phase of NGS. This PCR step ensures that there is sufficient amount of DNA to be sequenced. However, the main strength of NGS<\/strong> lies in its ability to simultaneously process millions to billions of DNA fragments at a time<\/strong>. This enables a wide range of applications, such as whole genome sequencing, profiling of expressed genes and even the analysis of epigenetic changes.<\/p>\n\n\n\n

NGS is sometimes compared to ‘high-volume PCR’<\/strong> because of its high-throughput nature, with millions of sequencing reactions taking place simultaneously. In reality, however, NGS provides more information than PCR, enabling more complex and comprehensive analysis of genetic information.<\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Next-generation sequencing (NGS) and polymerase chain reaction (PCR) are both techniq…<\/p>\n","protected":false},"author":5,"featured_media":1980,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[33],"tags":[39],"class_list":{"0":"post-2584","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-genetic-testing-en","8":"tag-expert-en","9":"article"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/2584","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/comments?post=2584"}],"version-history":[{"count":1,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/2584\/revisions"}],"predecessor-version":[{"id":2587,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/2584\/revisions\/2587"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media\/1980"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media?parent=2584"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/categories?post=2584"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/tags?post=2584"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}