{"id":2620,"date":"2024-08-30T23:58:51","date_gmt":"2024-08-30T14:58:51","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/nippt\/%e7%b2%be%e6%b6%b2%e3%81%8b%e3%82%89%e5%87%ba%e7%94%9f%e5%89%8d%e8%a6%aa%e5%ad%90%e9%91%91%e5%ae%9a%e3%82%92%e8%a1%8c%e3%81%86\/"},"modified":"2024-10-07T17:36:46","modified_gmt":"2024-10-07T08:36:46","slug":"%e7%b2%be%e6%b6%b2%e3%81%8b%e3%82%89%e5%87%ba%e7%94%9f%e5%89%8d%e8%a6%aa%e5%ad%90%e9%91%91%e5%ae%9a%e3%82%92%e8%a1%8c%e3%81%86","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/nippt\/%e7%b2%be%e6%b6%b2%e3%81%8b%e3%82%89%e5%87%ba%e7%94%9f%e5%89%8d%e8%a6%aa%e5%ad%90%e9%91%91%e5%ae%9a%e3%82%92%e8%a1%8c%e3%81%86\/?lang=en","title":{"rendered":"Prenatal paternity testing from semen."},"content":{"rendered":"\n

Methods of DNA extraction from condom semen.<\/strong><\/h2>\n\n\n\n

1. Sample collection<\/strong>:<\/h4>\n\n\n\n

Semen is carefully collected from used condoms<\/strong>. Sample handling at this stage is very important as semen contains male genetic information. Try to collect as much semen as possible and place it in a clean container to avoid contamination.<\/strong><\/p>\n\n\n\n

2. Cell degradation<\/strong>:<\/h4>\n\n\n\n

Collected semen contains a large number of sperm. Enzymes such as proteinase K<\/strong> are used to break down the sperm cell membranes and extract the DNA inside. At this stage, processing is often carried out in a temperature-controlled incubator, which allows DNA to be extracted efficiently<\/strong>.<\/p>\n\n\n\n

3. DNA purification:<\/strong><\/h4>\n\n\n\n

After DNA<\/strong> is extracted from semen, DNA purification<\/strong> is performed to remove other cellular components and impurities. Typically, phenol-chloroform extraction or silica column purification methods<\/strong> are used. This yields pure DNA, which is then used for subsequent analysis.<\/p>\n\n\n\n

4. PCR amplification:<\/strong><\/h4>\n\n\n\n

If the DNA sample obtained is small, the DNA is amplified using the polymerase chain reaction (<\/strong> PCR); PCR is an important technique for replicating a particular DNA region thousands of times to ensure the required quantity for analysis.<\/p>\n\n\n\n

5. DNA analysis<\/strong>:<\/h4>\n\n\n\n

Amplified DNA is used for genetic analysis and DNA profiling. This involves analyzing the DNA pattern of a particular individual<\/strong>, confirming parent-child relationships and personal identification. In forensic science, semen DNA is often used in criminal investigations and plays an important role as evidence.<\/p>\n\n\n\n

Relationship with prenatal paternity testing.<\/strong><\/h2>\n\n\n\n

Prenatal paternity testing<\/strong> is carried out while the fetus is still inside the mother’s body<\/strong> in order to establish the parent-child relationship with the prospective father. DNA extraction from condoms and prenatal paternity testing have several similarities and differences.<\/p>\n\n\n\n

Common denominators<\/strong>:<\/p>\n\n\n\n