{"id":2630,"date":"2024-08-30T23:53:24","date_gmt":"2024-08-30T14:53:24","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/nippt\/%e6%af%9b%e9%ab%aa%e3%81%8b%e3%82%89%e3%81%aedna%e6%8a%bd%e5%87%ba%e3%81%a7%e5%87%ba%e7%94%9f%e5%89%8d%e8%a6%aa%e5%ad%90%e9%91%91%e5%ae%9a%e3%82%92%e8%a1%8c%e3%81%86%e3%80%82\/"},"modified":"2024-10-07T17:47:39","modified_gmt":"2024-10-07T08:47:39","slug":"%e6%af%9b%e9%ab%aa%e3%81%8b%e3%82%89%e3%81%aedna%e6%8a%bd%e5%87%ba%e3%81%a7%e5%87%ba%e7%94%9f%e5%89%8d%e8%a6%aa%e5%ad%90%e9%91%91%e5%ae%9a%e3%82%92%e8%a1%8c%e3%81%86%e3%80%82","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/nippt\/%e6%af%9b%e9%ab%aa%e3%81%8b%e3%82%89%e3%81%aedna%e6%8a%bd%e5%87%ba%e3%81%a7%e5%87%ba%e7%94%9f%e5%89%8d%e8%a6%aa%e5%ad%90%e9%91%91%e5%ae%9a%e3%82%92%e8%a1%8c%e3%81%86%e3%80%82\/?lang=en","title":{"rendered":"DNA extraction from hair for prenatal parentage testing."},"content":{"rendered":"\n

Methods of DNA extraction from hair follicles.<\/strong><\/h3>\n\n\n\n

The extraction of DNA from hair follicles<\/strong> is used in a variety of situations, including criminal investigations and to confirm parent-child relationships. The following is the general procedure.<\/p>\n\n\n\n

    \n
  1. Sample collection<\/strong>: the hair is pulled out and the hair follicles<\/strong> at its base are sampled. These hair roots are rich in DNA and are suitable for testing.<\/li>\n\n\n\n
  2. Cytolysis<\/strong>: in order to extract DNA from the hair follicles, the cells must first be degraded. This is done using the enzyme proteinase K, which<\/strong> breaks down the cell membrane and cell nucleus, releasing the DNA. This process usually takes place in a temperature-controlled incubator for several hours.<\/li>\n\n\n\n
  3. DNA purification<\/strong>: the DNA released from cells is mixed with other cellular components. Methods such as phenol-chloroform extraction and silica column purification<\/strong> are used to purify this DNA. This yields pure DNA.<\/li>\n\n\n\n
  4. PCR amplification<\/strong>: if the DNA obtained is in small quantities, it is amplified to ensure that it is sufficient for analysis. Polymerase chain reaction (PCR)<\/strong> is used to replicate specific regions of DNA<\/strong> and increase them to the quantity required for testing.<\/li>\n\n\n\n
  5. Analysis<\/strong>: finally, purified DNA is used for genetic analysis and DNA profiling<\/strong>. This enables confirmation of parent-child relationships and personal identification.<\/li>\n<\/ol>\n\n\n\n

    Relationship with prenatal paternity testing.<\/strong><\/h3>\n\n\n\n

    Prenatal paternity testing<\/strong> confirms the parent-child relationship with the potential father while the fetus is still inside the mother’s body<\/strong>. This is done by using maternal blood, amniotic fluid or chorionic villi to<\/strong> extract the fetus’ DNA and compare it with that of the father. Non-invasive methods that analyze free fetal DNA (cfDNA) in maternal blood<\/strong> are commonly used.<\/p>\n\n\n\n

    Prenatal paternity testing and DNA extraction from hair follicles have<\/strong> one thing in common<\/strong>: both use DNA to confirm parent-child relationships. Both methods have in common that DNA is extracted and analyzed using techniques such as PCR. However, the specimens used are different. As prenatal paternity testing<\/strong> involves the DNA of the fetus, methods<\/strong> are used to collect DNA directly from the fetus (amniotic fluid or chorionic villus sampling)<\/strong> or indirectly from the mother’s blood (cfDNA)<\/strong>.<\/p>\n\n\n\n

    DNA extraction from hair follicles is primarily used for postnatal paternity testing. Unlike prenatal paternity testing, DNA from hair follicles is obtained from a direct sample and is therefore non-invasive yet highly accurate<\/strong>. Prenatal paternity testing<\/strong>, on the other hand, requires careful procedures to ensure that the fetus’ DNA is safely extracted from the mother’s body. Non-invasive NIPPT<\/a><\/strong> has become an important technique for confirming parent-child relationships without any risk to the mother or the fetus.<\/p>\n\n\n\n

    Conclusion<\/strong><\/h3>\n\n\n\n

    DNA extraction from hair follicles is a very effective method in confirming parent-child relationships and personal identification, and is widely used in criminal investigations and forensic medicine. Mean while, prenatal paternity testing is<\/strong> developing methods to confirm parent-child relationships using fetal DNA<\/strong>, with the non-invasive NIPPT<\/a><\/strong> receiving particular attention. With the development of these technologies, we can look forward to a future where our lives are safer and where individual genetic information is used appropriately. HIRO Clinic offers DNA collection from hair follicles.<\/strong> Please understand that this costs an additional JPY 33 000.<\/p>\n","protected":false},"excerpt":{"rendered":"

    Methods of DNA extraction from hair follicles. The extraction of DNA from hair follic…<\/p>\n","protected":false},"author":5,"featured_media":2020,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[34,39],"class_list":{"0":"post-2630","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-nippt-en","8":"tag-beginner-en","9":"tag-expert-en","10":"article"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/2630","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/comments?post=2630"}],"version-history":[{"count":3,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/2630\/revisions"}],"predecessor-version":[{"id":5607,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/2630\/revisions\/5607"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media\/2020"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media?parent=2630"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/categories?post=2630"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/tags?post=2630"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}