{"id":5054,"date":"2024-10-03T19:49:00","date_gmt":"2024-10-03T10:49:00","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/nippt\/parentage-testing-str-to-ff-method\/"},"modified":"2024-10-04T16:07:49","modified_gmt":"2024-10-04T07:07:49","slug":"parentage-testing-str-to-ff-method","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/nippt\/parentage-testing-str-to-ff-method\/?lang=en","title":{"rendered":"Methods for determining FF (Fetal Fractionation Rate) from paternity tested STRs."},"content":{"rendered":"\n<h2 class=\"wp-block-heading\">How to obtain FF (Fetal Fraction Fractionation Rate) from STR analysis<\/h2>\n\n\n\n<p><a href=\"https:\/\/www.hiro-clinic.or.jp\/nippt\/accuracy\/\">STR<\/a> (Short Tandem Repeat) is a method for analyzing the repeat pattern of a specific region of DNA in paternity testing.<\/p>\n\n\n<div class=\"custom-html\"><div class=\"short-code\">\n<p><span>Reliable domestic inspections<\/span><br \/>Available at a clinic near you nationwide<\/p>\n<div class=\"short-button\">\n   <a href=\"https:\/\/mypage-nippt.hiro-clinic.or.jp\/register\">Make a reservation now!<\/a>\n <\/div>\n<\/div>\n<style>\n.short-code{\n text-align: center;\n background-color: var(--stk-editor-color3);\n padding: 20px 0;\n border-top: dashed 2px #ffbb00;\n border-bottom: dashed 2px #ffbb00;\n}\n.short-code p{\n margin-bottom: 5px;\n font-weight: 600;\n}\n.short-code p span{\n position: relative;\n background-image: linear-gradient(to bottom, rgba(255, 255, 255, 0) 70%, var(--stk-maker-yellow, \"#ffd5d5\") 30%);\n}\n.short-code p span::before, .short-code p span::after{\n content: \"\";\n display: inline-block;\n position: absolute;\n bottom: 0;\n background-color: #333;\n width: 1px;\n height: 16px;\n}\n.short-code p span::before{\n transform: rotate(-18deg);\n left: -10px;\n}\n.short-code p span::after{\n right: -10px;\n transform: rotate(18deg);\n}\n.short-button a {\n position: relative;\n display: flex;\n justify-content: space-around;\n align-items: center;\n margin: auto;\n max-width: 250px;\n padding: 10px 25px;\n color: #333;\n font-family: \"Noto Sans Japanese\";\n text-decoration: none;\n color: #fff;\n line-height: 1.8;\n transition: 0.3s ease-in-out;\n font-weight: 700;\n background: #ff8300;\n border-radius: 9999px;\n border-bottom: solid 5px #af4f00;\n}\n.short-button a:hover {\n border-bottom: solid 2px #af4f00;\n transform: translateY(3px);\n}\n<\/style>\n<\/div>\n\n\n\n<h3 class=\"wp-block-heading\">Procedure:<\/h3>\n\n\n\n<p><strong>Separation of maternal and fetal cfDNA<\/strong>:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>cfDNA is collected from the mother&#8217;s blood to distinguish between DNA of maternal and fetal origin. Fetal-derived DNA is less abundant than maternal-derived DNA because it flows mainly from the placenta into the mother&#8217;s blood.<\/li>\n<\/ul>\n\n\n\n<p><strong>\u7279\u5b9a\u306eSTR\u30de\u30fc\u30ab\u30fc\u306e\u89e3\u6790<\/strong>\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.hiro-clinic.or.jp\/nippt\/accuracy\/\">STR<\/a> markers are short repeating sequences in specific gene regions that have different patterns in different individuals. DNA from the mother and presumptive father are compared and the <a href=\"https:\/\/www.hiro-clinic.or.jp\/nippt\/accuracy\/\">STR<\/a> profile of the fetus is matched to that of the mother.<\/li>\n<\/ul>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"800\" height=\"533\" src=\"\/nippt\/wp-content\/uploads\/2024\/08\/PCRset12007_TP_V4.jpg\" alt=\"\u89aa\u5b50\u9451\u5b9a\u3000NIPPT\u3000DNA\u9451\u5b9a\u3000\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u9023\u9396\u53cd\u5fdc\u3000\uff08PCR\uff09\" class=\"wp-image-2032\" srcset=\"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/08\/PCRset12007_TP_V4.jpg 800w, https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/08\/PCRset12007_TP_V4-300x200.jpg 300w, https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/08\/PCRset12007_TP_V4-768x512.jpg 768w, https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/08\/PCRset12007_TP_V4-485x323.jpg 485w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n<p><strong>Calculation of Fetal Fractionation Rate (FF)<\/strong>:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>The peak ratios of common and different <a href=\"https:\/\/www.hiro-clinic.or.jp\/nippt\/accuracy\/\">STR<\/a> markers of the mother and fetus are used to calculate the fraction of DNA of fetal origin. Specifically, the peak intensities of maternal cfDNA and fetal cfDNA are compared, and the fetal fractionation rate is derived from the ratios. Usually, a highly reliable result is obtained when the fractionation rate of DNA of fetal origin is 4% or higher.<\/li>\n<\/ul>\n\n\n\n<p><strong>\u7d50\u679c\u306e\u78ba\u8a8d<\/strong>\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><\/li>\n<\/ol>\n\n\n\n<ul class=\"wp-block-list\">\n<li>After the fetal fractionation rate is calculated, the reliability of the results of the paternity test is confirmed. If the fractionation rate is low, retesting may be necessary.<\/li>\n<\/ul>\n\n\n<div class=\"custom-html\"><div class=\"short-code\">\n<p><span>Reliable domestic inspections<\/span><br \/>Available at a clinic near you nationwide<\/p>\n<div class=\"short-button\">\n   <a href=\"https:\/\/mypage-nippt.hiro-clinic.or.jp\/register\">Make a reservation now!<\/a>\n <\/div>\n<\/div>\n<style>\n.short-code{\n text-align: center;\n background-color: var(--stk-editor-color3);\n padding: 20px 0;\n border-top: dashed 2px #ffbb00;\n border-bottom: dashed 2px #ffbb00;\n}\n.short-code p{\n margin-bottom: 5px;\n font-weight: 600;\n}\n.short-code p span{\n position: relative;\n background-image: linear-gradient(to bottom, rgba(255, 255, 255, 0) 70%, var(--stk-maker-yellow, \"#ffd5d5\") 30%);\n}\n.short-code p span::before, .short-code p span::after{\n content: \"\";\n display: inline-block;\n position: absolute;\n bottom: 0;\n background-color: #333;\n width: 1px;\n height: 16px;\n}\n.short-code p span::before{\n transform: rotate(-18deg);\n left: -10px;\n}\n.short-code p span::after{\n right: -10px;\n transform: rotate(18deg);\n}\n.short-button a {\n position: relative;\n display: flex;\n justify-content: space-around;\n align-items: center;\n margin: auto;\n max-width: 250px;\n padding: 10px 25px;\n color: #333;\n font-family: \"Noto Sans Japanese\";\n text-decoration: none;\n color: #fff;\n line-height: 1.8;\n transition: 0.3s ease-in-out;\n font-weight: 700;\n background: #ff8300;\n border-radius: 9999px;\n border-bottom: solid 5px #af4f00;\n}\n.short-button a:hover {\n border-bottom: solid 2px #af4f00;\n transform: translateY(3px);\n}\n<\/style>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Fetal fractionation rate criteria:<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>It is usually recommended that the fetal fractionation rate be at least 4% for non-invasive paternity testing performed <strong>after the sixth week of pregnancy<\/strong>.This ensures that the fetal DNA is sufficiently contained to provide a reliable test result.<\/li>\n<\/ul>\n\n\n\n<p>Fetal fractionation rate is an important indicator to ensure the reliability of the paternity test, and this value must be adequate to obtain accurate test results.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>How to obtain FF (Fetal Fraction Fractionation Rate) from STR analysis STR (Short Tan&#8230;<\/p>\n","protected":false},"author":5,"featured_media":2001,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[79],"tags":[73,69,39,91,92,80,74,70,71],"class_list":{"0":"post-5054","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-str-en","8":"tag-dna_profiling-en","9":"tag-dna_test-en","10":"tag-expert-en","11":"tag-regnancy-en","12":"tag-mother-en","13":"tag-forensic_science-en","14":"tag-accuracy-en","15":"tag-result-en","16":"tag-parent_child-en","17":"article"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/5054","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/comments?post=5054"}],"version-history":[{"count":2,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/5054\/revisions"}],"predecessor-version":[{"id":5086,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/5054\/revisions\/5086"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media\/2001"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media?parent=5054"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/categories?post=5054"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/tags?post=5054"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}