{"id":6197,"date":"2024-10-19T22:06:00","date_gmt":"2024-10-19T13:06:00","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/nippt\/nextseq-high-throughput-genomics\/"},"modified":"2024-10-23T12:49:30","modified_gmt":"2024-10-23T03:49:30","slug":"nextseq-high-throughput-genomics","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/nippt\/nextseq-high-throughput-genomics\/?lang=zh-hans","title":{"rendered":"\u4ec0\u4e48\u662f NextSeq\uff1f"},"content":{"rendered":"\n\n\n\n<h3 class=\"wp-block-heading\">1. NextSeq \u7684\u7279\u70b9<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\">\u9ad8\u4ea7\u91cf<\/h4>\n\n\n\n<p>NextSeq \u63d0\u4f9b\u9ad8\u901a\u91cf\u6570\u636e\u751f\u6210\uff0c\u5355\u6b21\u8fd0\u884c\u53ef\u751f\u6210<strong>\u9ad8\u8fbe 120 Gb \u7684<\/strong>\u6570\u636e\u3002\u8fd9\u4f7f\u5b83\u9002\u7528\u4e8e\u9700\u8981\u5927\u91cf\u6570\u636e\u7684\u9879\u76ee\uff0c<strong>\u5982\u5168\u57fa\u56e0\u7ec4\u5206\u6790\u548c\u5927\u89c4\u6a21 RNA \u6d4b\u5e8f<\/strong>\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u7075\u6d3b\u7684\u541e\u5410\u91cf\u9009\u9879<\/h4>\n\n\n\n<p>NextSeq \u63d0\u4f9b<strong>\u4e24\u79cd\u5361\u5323\u9009\u9879<\/strong>\u3002\u60a8\u53ef\u4ee5\u6839\u636e\u9879\u76ee\u7684\u89c4\u6a21\u9009\u62e9<strong>\u9ad8\u8f93\u51fa<\/strong>\u6216<strong>\u4e2d\u8f93\u51fa\u6a21\u5f0f<\/strong>\uff0c\u5e76\u8c03\u6574\u751f\u6210\u7684\u6570\u636e\u91cf\u548c\u8fd0\u884c\u65f6\u95f4\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u9ad8\u8f93\u51fa\u6a21\u5f0f<\/strong>\uff1a\u53ef\u751f\u6210\u9ad8\u8fbe 120 Gb \u7684\u6570\u636e\u91cf\u548c\u9ad8\u8fbe 800 M \u8bfb\u6570\uff08100 \u78b1\u57fa\u5bf9 (bp) \u6210\u5bf9\u672b\u7aef\u8bfb\u6570\uff09\u3002<\/li>\n\n\n\n<li><strong>\u4e2d\u95f4\u8f93\u51fa\u6a21\u5f0f<\/strong>\uff1a\u4ea7\u751f\u591a\u8fbe 39 Gb \u7684\u6570\u636e\u548c\u591a\u8fbe 260 M \u4e2a 100 \u78b1\u57fa\u5bf9\u914d\u5bf9\u7aef\u8bfb\u6570\u3002<\/li>\n<\/ul>\n\n\n\n<h4 class=\"wp-block-heading\">\u5feb\u901f\u5206\u6790<\/h4>\n\n\n\n<p>\u4e0e\u4f20\u7edf\u7684 Illumina \u6d4b\u5e8f\u4eea\u76f8\u6bd4\uff0cNextSeq \u6d4b\u5e8f\u901f\u5ea6\u6781\u5feb\uff1a\u4e00\u6b21\u8fd0\u884c\u53ef\u5728\u4e00\u4e24\u5929\u5185\u63d0\u4f9b\u5206\u6790\u7ed3\u679c\uff0c\u56e0\u6b64\u9002\u5408\u65f6\u95f4\u7d27\u8feb\u7684\u9879\u76ee\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u53ef\u5ef6\u957f\u7684\u5bfc\u7ebf\u957f\u5ea6<\/h4>\n\n\n\n<p>NextSeq \u63d0\u4f9b\u7075\u6d3b\u7684\u8bfb\u53d6\u957f\u5ea6\uff0c\u6700\u9ad8\u53ef\u8fbe<strong>150 \u78b1\u57fa\u5bf9 (bp)<\/strong>\u3002\u8fd9\u4f7f\u5f97\u77ed\u8bfb\u6570\u548c\u957f\u8bfb\u6570\u90fd\u80fd\u7528\u4e8e\u5206\u6790\u3002<\/p>\n\n\n\n\n\n\n<h3 class=\"wp-block-heading\">2. NextSeq \u6280\u672f<\/h3>\n\n\n\n<h4 class=\"wp-block-heading\">\u5408\u6210\u6d4b\u5e8f\uff08SBS\uff09\u6280\u672f<\/h4>\n\n\n\n<p>\u4e0e\u6240\u6709 Illumina \u6d4b\u5e8f\u4eea\u4e00\u6837\uff0cNextSeq \u4e5f\u91c7\u7528\u4e86<strong>\u5408\u6210\u6d4b\u5e8f\uff08SBS<\/strong>\uff09\u6280\u672f\uff0c\u5728\u8fd9\u9879\u6280\u672f\u4e2d\uff0c\u8367\u5149\u6807\u8bb0\u7684\u6838\u82f7\u9178\u88ab\u9010\u4e2a\u78b1\u57fa\u6574\u5408\u5230 DNA \u4e2d\uff0c\u5e76\u5b9e\u65f6\u68c0\u6d4b\u8fd9\u4e00\u8fc7\u7a0b\uff0c\u4ece\u800c\u786e\u5b9a DNA \u7684\u5e8f\u5217\u3002\u8fd9\u4e00\u8fc7\u7a0b\u88ab\u5b9e\u65f6\u68c0\u6d4b\u5e76\u786e\u5b9a\u5e8f\u5217\u3002\u8fd9\u5c31\u5b9e\u73b0\u4e86\u9ad8\u7cbe\u5ea6\u548c\u9ad8\u901a\u91cf\u6d4b\u5e8f\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u53cc\u901a\u9053\u5316\u5b66<\/h4>\n\n\n\n<p>NextSeq \u4f7f\u7528 Illumina \u7684<strong>\u53cc\u901a\u9053<\/strong>\u5316\u5b66\u65b9\u6cd5\uff0c\u6bd4\u4f20\u7edf\u7684\u56db\u901a\u9053\u65b9\u6cd5\u8bfb\u53d6\u78b1\u57fa\u7684\u901f\u5ea6\u66f4\u5feb\u3002\u53cc\u901a\u9053\u5316\u5b66\u65b9\u6cd5\u4f7f\u7528\u4e24\u4e2a\u8367\u5149\u4fe1\u53f7\u6765\u8bc6\u522b\u56db\u4e2a\u4e0d\u540c\u7684\u78b1\u57fa\uff0c\u4ece\u800c\u52a0\u5feb\u4e86\u6d4b\u5e8f\u5468\u671f\u3002\u8be5\u7cfb\u7edf\u8fd8\u63d0\u4f9b\u4ee5\u4e0b\u683c\u5f0f\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u5bf9\u7aef\u5e8f\u5217<\/h4>\n\n\n\n<p>NextSeq \u652f\u6301<strong>\u6210\u5bf9\u7aef\u6d4b\u5e8f<\/strong>\uff08\u53cc\u5411\u6d4b\u5e8f\uff09\uff0c\u901a\u8fc7\u5bf9 DNA \u7247\u6bb5\u7684\u4e24\u7aef\u8fdb\u884c\u6d4b\u5e8f\uff0c\u63d0\u9ad8\u4e86\u57fa\u56e0\u7ec4\u5206\u6790\u7684\u51c6\u786e\u6027\u3002\u8fd9\u6837\u5c31\u80fd\u68c0\u6d4b\u590d\u6742\u7684\u7a81\u53d8\uff0c\u5982\u7ed3\u6784\u53d8\u5f02\u3001\u63d2\u5165\u548c\u7f3a\u5931\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">\u96c6\u6210\u6d41\u52a8\u6c60<\/h4>\n\n\n\n<p>NextSeq \u4f7f\u7528<strong>\u96c6\u6210\u7684\u6d41\u52a8\u6c60<\/strong>\uff0c\u5bf9\u6570\u4ebf\u4e2a\u7c07\u8fdb\u884c\u5e76\u884c\u6d4b\u5e8f\u3002\u8fd9\u6837\u5c31\u80fd\u540c\u65f6\u5bf9\u5927\u91cf DNA \u7247\u6bb5\u8fdb\u884c\u6d4b\u5e8f\u3002<\/p>\n\n\n\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"800\" height=\"533\" src=\"\/nippt\/wp-content\/uploads\/2024\/09\/takaomiudsc_0100-2739_TP_V4-1.jpg\" alt=\"\u89aa\u5b50\u9451\u5b9a\u3000NIPPT\u3000DNA\u9451\u5b9a\u3000\u533b\u7642\u884c\u70ba\" class=\"wp-image-2670\" srcset=\"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/09\/takaomiudsc_0100-2739_TP_V4-1.jpg 800w, https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/09\/takaomiudsc_0100-2739_TP_V4-1-300x200.jpg 300w, https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/09\/takaomiudsc_0100-2739_TP_V4-1-768x512.jpg 768w, https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-content\/uploads\/2024\/09\/takaomiudsc_0100-2739_TP_V4-1-485x323.jpg 485w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/><\/figure><\/div>\n\n\n<h3 class=\"wp-block-heading\">3. NextSeq \u7684\u5e94\u7528<\/h3>\n\n\n\n<p>NextSeq \u662f<strong>\u5927\u4e2d\u578b\u6d4b\u5e8f\u9879\u76ee<\/strong>\u7684\u7406\u60f3\u9009\u62e9\u3002\u5b83\u5c24\u5176\u5e7f\u6cdb\u7528\u4e8e\u9700\u8981\u5feb\u901f\u83b7\u53d6\u5927\u91cf\u6570\u636e\u7684\u7814\u7a76\u3002NextSeq \u7684\u4e3b\u8981\u5e94\u7528\u5982\u4e0b\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">3.1 \u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\uff08WGS\uff09<\/h4>\n\n\n\n<p>NextSeq \u7528\u4e8e<strong>\u4eba\u7c7b\u57fa\u56e0\u7ec4\u548c<\/strong>\u5176\u4ed6\u751f\u7269\u4f53<strong>\u7684\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f<\/strong>\uff1b\u4e00\u6b21\u8fd0\u884c\u5373\u53ef\u5206\u6790\u6574\u4e2a\u4eba\u7c7b\u57fa\u56e0\u7ec4\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u764c\u75c7\u7814\u7a76\u3001\u9057\u4f20\u75be\u75c5\u5206\u6790\u548c\u8fdb\u5316\u7814\u7a76\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">3.2 \u5916\u663e\u5b50\u7ec4\u6d4b\u5e8f<\/h4>\n\n\n\n<p>NextSeq \u8fd8\u9002\u7528\u4e8e<strong>\u5916\u663e\u5b50\u7ec4\u6d4b\u5e8f<\/strong>\uff08\u4ec5\u5bf9\u57fa\u56e0\u7f16\u7801\u533a\u6d4b\u5e8f\uff09\uff0c\u53ef\u5feb\u901f\u83b7\u53d6\u4e0e\u7279\u5b9a\u75be\u75c5\u548c\u57fa\u56e0\u53d8\u5f02\u6709\u5173\u7684\u6570\u636e\u3002\u5bf9\u4e8e\u4eba\u7c7b\u5916\u663e\u5b50\u7ec4\u5206\u6790\uff0c\u53ef\u4ee5\u540c\u65f6\u5904\u7406\u6570\u767e\u4e2a\u6837\u672c\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">3.3 \u8f6c\u5f55\u7ec4\u5206\u6790\uff08RNA-seq\uff09<\/h4>\n\n\n\n<p>NextSeq<strong>\u8fd8<\/strong>\u5e7f\u6cdb\u5e94\u7528 <strong>RNA \u6d4b\u5e8f\uff08RNA-seq\uff09<\/strong>\u8fdb\u884c<strong>\u57fa\u56e0\u8868\u8fbe\u5206\u6790<\/strong>\u3002\u8fd9\u6837\u5c31\u80fd\u5bf9\u7ec4\u7ec7\u548c\u7ec6\u80de\u4e2d\u8868\u8fbe\u7684\u57fa\u56e0\u8fdb\u884c\u5168\u9762\u7814\u7a76\uff0c\u5e76\u5206\u6790\u4e0e\u75be\u75c5\u548c\u73af\u5883\u53cd\u5e94\u76f8\u5173\u7684\u8868\u8fbe\u53d8\u5f02\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">3.4 \u591a\u76ee\u6807\u6d4b\u5e8f<\/h4>\n\n\n\n<p>NextSeq \u8fd8\u53ef\u7528\u4e8e\u9ad8\u901a\u91cf\u9776\u5411\u6d4b\u5e8f\uff0c\u4ee5<strong>\u5168\u9762\u68c0\u6d4b<\/strong>\u764c\u75c7\u7814\u7a76\u548c\u9057\u4f20\u75be\u75c5\u7814\u7a76\u4e2d\u7684<strong>\u57fa\u56e0\u7a81\u53d8<\/strong>\u3002\u5b83\u5728\u5206\u6790\u7279\u5b9a\u57fa\u56e0\u7ec4\u6216\u540c\u65f6\u5bf9\u5927\u91cf\u6837\u672c\u8fdb\u884c\u6d4b\u5e8f\u65f6\u975e\u5e38\u9ad8\u6548\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\">3.5 \u5fae\u751f\u7269\u57fa\u56e0\u7ec4\u5206\u6790<\/h4>\n\n\n\n<p>NextSeq<strong>\u8fd8<\/strong>\u53ef\u7528\u4e8e<strong>\u5fae\u751f\u7269\u7684\u5168\u57fa\u56e0\u7ec4\u548c\u5143\u57fa\u56e0\u7ec4\u5206\u6790<\/strong>\uff0c\u9002\u7528\u4e8e\u5206\u6790\u73af\u5883\u4e2d\u7684\u5404\u79cd\u5fae\u751f\u7269\u7fa4\u843d\u548c\u9274\u5b9a\u4f20\u67d3\u75c5\u75c5\u539f\u4f53\u3002<\/p>\n\n\n\n\n\n\n<h3 class=\"wp-block-heading\">4. NextSeq \u7684\u4f18\u52bf<\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>\u541e\u5410\u91cf\u7075\u6d3b\u6027<\/strong>\uff1aNextSeq \u53ef\u6839\u636e\u9879\u76ee\u89c4\u6a21\u914d\u7f6e\u4e3a\u9ad8\u6216\u4e2d\u8f93\u51fa\uff0c\u4f7f\u60a8\u53ef\u4ee5\u7075\u6d3b\u5730\u8fdb\u884c\u5c0f\u89c4\u6a21\u9776\u5411\u5206\u6790\u5230\u5927\u89c4\u6a21\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u3002<\/li>\n\n\n\n<li><strong>\u77ed\u65f6\u95f4\u5185<\/strong>\u83b7\u53d6<strong>\u5927\u91cf\u6570\u636e<\/strong>\uff1a\u5feb\u901f\u6d4b\u5e8f\u8fc7\u7a0b\u53ef\u5728\u77ed\u65f6\u95f4\u5185\u83b7\u53d6\u5927\u91cf\u5e8f\u5217\u6570\u636e\uff0c\u56e0\u6b64\u9002\u5408\u65f6\u95f4\u7d27\u8feb\u7684\u9879\u76ee\u3002<\/li>\n\n\n\n<li><strong>\u53cb\u597d\u7684\u7528\u6237\u754c\u9762<\/strong>\uff1aNextSeq \u548c\u6240\u6709 Illumina \u6d4b\u5e8f\u4eea\u4e00\u6837\uff0c\u62e5\u6709\u76f4\u89c2\u7684\u8f6f\u4ef6\u8bbe\u8ba1\uff0c\u4fbf\u4e8e\u521d\u5b66\u8005\u4f7f\u7528\u3002<\/li>\n\n\n\n<li><strong>\u9ad8\u5ea6\u51c6\u786e\u7684\u6570\u636e<\/strong>\uff1aIllumina SBS \u6280\u672f\u548c\u53cc\u901a\u9053\u5316\u5b66\u6280\u672f\u53ef\u63d0\u4f9b\u9ad8\u5ea6\u51c6\u786e\u53ef\u9760\u7684\u6570\u636e\u3002\u8fd9\u5bf9\u4e8e\u68c0\u6d4b\u590d\u6742\u7684\u57fa\u56e0\u7ec4\u53d8\u5f02\u5c24\u5176\u6709\u7528\u3002<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\">\u6458\u8981<\/h3>\n\n\n\n<p><strong>NextSeq<\/strong>\u662f\u4e00\u6b3e\u9002\u7528\u4e8e<strong>\u5927\u4e2d\u578b\u6d4b\u5e8f\u9879\u76ee<\/strong>\u7684\u65b0\u4e00\u4ee3<strong>\u6d4b\u5e8f<\/strong>\u4eea\uff0c\u5e7f\u6cdb\u7528\u4e8e<strong>\u5168\u57fa\u56e0\u7ec4\u5206\u6790<\/strong>\u3001<strong>RNA \u6d4b\u5e8f<\/strong>\u548c<strong>\u5916\u663e\u5b50\u7ec4\u6d4b\u5e8f<\/strong>\u3002\u5b83\u5e7f\u6cdb\u7528\u4e8e\u5168\u57fa\u56e0\u7ec4\u5206\u6790\u3001RNA \u6d4b\u5e8f\u548c\u5916\u663e\u5b50\u7ec4\u6d4b\u5e8f\u3002\u5b83\u4eec\u53ef\u5b9e\u73b0<strong>\u9ad8\u901a\u91cf<\/strong>\u548c<strong>\u5feb\u901f\u6570\u636e\u91c7\u96c6<\/strong>\uff0c\u7279\u522b\u9002\u7528\u4e8e\u9700\u8981\u5927\u91cf\u6570\u636e\u7684\u7814\u7a76\u548c\u4e34\u5e8a\u5e94\u7528\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>1. NextSeq \u7684\u7279\u70b9 \u9ad8\u4ea7\u91cf NextSeq \u63d0\u4f9b\u9ad8\u901a\u91cf\u6570\u636e\u751f\u6210\uff0c\u5355\u6b21\u8fd0\u884c\u53ef\u751f\u6210\u9ad8\u8fbe 120 Gb \u7684\u6570\u636e\u3002\u8fd9\u4f7f\u5b83\u9002\u7528\u4e8e\u9700\u8981\u5927\u91cf\u6570\u636e\u7684\u9879\u76ee\uff0c\u5982\u5168\u57fa\u56e0\u7ec4\u5206\u6790\u548c\u5927\u89c4\u6a21&#8230;<\/p>\n","protected":false},"author":5,"featured_media":2033,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[31],"tags":[81,78,40,66,83,67,68],"class_list":{"0":"post-6197","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-genetic-testing-zh-hans","8":"tag-dna_profiling-zh-hans","9":"tag-dna_test-zh-hans","10":"tag-expert-zh-hans","11":"tag-accuracy-zh-hans","12":"tag-result-zh-hans","13":"tag-genetic_testing-zh-hans","14":"tag-analysis-zh-hans","15":"article"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6197","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/comments?post=6197"}],"version-history":[{"count":2,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6197\/revisions"}],"predecessor-version":[{"id":6204,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6197\/revisions\/6204"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media\/2033"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media?parent=6197"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/categories?post=6197"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/tags?post=6197"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}