Flexible throughput options<\/h4>\n\n\n\n
NextSeq offers two cartridge options<\/strong>. This allows you to choose either High Output<\/strong> or Mid Output mode<\/strong>, depending on the size of your project, and adjust the amount of data generated and run time.<\/p>\n\n\n\n NextSeq is extremely fast sequencing compared to traditional Illumina sequencers; a single run provides analysis results within one to two days, making it ideal for time-sensitive projects.<\/p>\n\n\n\n NextSeq offers flexible read lengths, up to 150 base pairs (bp)<\/strong>. This allows for either short or long reads to be used for analysis.<\/p>\n\n\n\n\n\n\n NextSeq, like all Illumina sequencers, employs Sequencing by Synthesis (SBS<\/strong> ) technology, a technique in which fluorescently labeled nucleotides are incorporated into DNA one nucleotide at a time and the process is detected and sequenced in real time. The SBS technology is a method of sequencing DNA by detecting the incorporation of fluorescently labeled nucleotides one base at a time in real time. This allows for highly accurate and high-throughput sequencing.<\/p>\n\n\n\n NextSeq uses Illumina’s 2-channel<\/strong> chemistry to read bases faster than traditional 4-channel methods. 2-channel chemistry uses two fluorescent signals to identify four different bases, which speeds up the sequencing cycle. <\/p>\n\n\n\n NextSeq supports paired-end sequencing<\/strong> (bidirectional sequencing), which improves the accuracy of genomic analysis by sequencing both ends of a DNA fragment. This enables the detection of complex mutations such as structural mutations, insertions, and deletions.<\/p>\n\n\n\n NextSeq uses an integrated flow cell<\/strong> and hundreds of millions of clusters are sequenced in parallel. This allows a huge number of DNA fragments to be sequenced simultaneously.<\/p>\n\n\n\n\n\n NextSeq is ideal for medium to large sequencing projects<\/strong>. It is especially widely used for studies that require rapid acquisition of large amounts of data. Below are the main applications of NextSeq.<\/p>\n\n\n\n NextSeq is used for whole genome sequencing of the human genome and<\/strong> other organisms; it can analyze the entire human genome in a single run and is widely used in cancer research, genetic disease analysis, and evolutionary research.<\/p>\n\n\n\n NextSeq is also suitable for exome sequencing<\/strong> (sequencing only the coding region of a gene), which allows rapid acquisition of data related to specific diseases or genetic mutations. For human exome analysis, hundreds of samples can be processed in parallel.<\/p>\n\n\n\n Gene expression analysis byRNA sequencing (RNA-seq)<\/strong> is also<\/strong> widely performed with NextSeq. This enables comprehensive examination of genes expressed in tissues and cells, and analysis of expression variations related to diseases and environmental responses.<\/p>\n\n\n\n NextSeq is also used for high-throughput targeted sequencing to comprehensively detect genetic mutations<\/strong> in cancer research and genetic disease studies. It is very efficient when analyzing specific gene panels or when sequencing a large number of samples at once.<\/p>\n\n\n\n NextSeq is also<\/strong> used for whole genome and metagenomic analysis of microorganisms<\/strong> and is suitable for profiling diverse microbial communities in the environment and identifying infectious disease pathogens.<\/p>\n\n\n\n\n\n\n NextSeq<\/strong> is a next-generation sequencer for medium to large sequencing projects<\/strong> and is widely used for whole genome analysis<\/strong>, RNA sequencing<\/strong>, and exome sequencing<\/strong>. It is widely used for whole genome analysis, RNA sequencing, and exome sequencing. High throughput<\/strong> and rapid data acquisition<\/strong> make it particularly suitable for research and clinical applications requiring large amounts of data.<\/p>\n","protected":false},"excerpt":{"rendered":" 1. features of NextSeq high throughput NextSeq offers high-throughput data generation…<\/p>\n","protected":false},"author":5,"featured_media":2034,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[33],"tags":[73,69,39,74,70,76,72],"class_list":{"0":"post-6198","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-genetic-testing-en","8":"tag-dna_profiling-en","9":"tag-dna_test-en","10":"tag-expert-en","11":"tag-accuracy-en","12":"tag-result-en","13":"tag-genetic_testing-en","14":"tag-analysis-en","15":"article"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6198","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/comments?post=6198"}],"version-history":[{"count":2,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6198\/revisions"}],"predecessor-version":[{"id":6201,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6198\/revisions\/6201"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media\/2034"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media?parent=6198"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/categories?post=6198"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/tags?post=6198"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}\n
Fast analysis<\/h4>\n\n\n\n
Extensible lead length<\/h4>\n\n\n\n
2. the NextSeq technology<\/h3>\n\n\n\n
Sequencing Bisynthesis (SBS) technology<\/h4>\n\n\n\n
2-Channel Chemistry<\/h4>\n\n\n\n
pair-end sequence<\/h4>\n\n\n\n
Integrated Flow Cell<\/h4>\n\n\n\n
![\"\u89aa\u5b50\u9451\u5b9a\u3000NIPPT\u3000DNA\u9451\u5b9a\u3000\u533b\u7642\u884c\u70ba\"](\"\/nippt\/wp-content\/uploads\/2024\/09\/takaomiudsc_0100-2739_TP_V4-1.jpg\")
<\/figure><\/div>\n\n\n3. Applications of NextSeq<\/h3>\n\n\n\n
3.1 Whole genome sequencing (WGS)<\/h4>\n\n\n\n
3.2 Exome sequencing<\/h4>\n\n\n\n
3.3 Transcriptome analysis (RNA-seq)<\/h4>\n\n\n\n
3.4 Multiplex Target Sequencing<\/h4>\n\n\n\n
3.5 Microbial Genome Analysis<\/h4>\n\n\n\n
4. Advantages of NextSeq<\/h3>\n\n\n\n
\n
summary<\/h3>\n\n\n\n