{"id":6208,"date":"2024-10-19T22:07:49","date_gmt":"2024-10-19T13:07:49","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/nippt\/novaseq-high-throughput-genomic-analysis\/"},"modified":"2024-10-23T13:01:05","modified_gmt":"2024-10-23T04:01:05","slug":"novaseq-high-throughput-genomic-analysis","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/nippt\/novaseq-high-throughput-genomic-analysis\/?lang=zh-hans","title":{"rendered":"\u4ec0\u4e48\u662f\u6700\u5148\u8fdb\u7684 Novaseq\uff1f"},"content":{"rendered":"\n

NovaSeq \u662f\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u7684\u9886\u5148\u578b\u53f7\uff0c\u63d0\u4f9b\u591a\u79cd\u6570\u636e\u541e\u5410\u91cf\u9009\u9879\uff0c\u7528\u4e8e\u5206\u6790\u5927\u91cf\u6837\u672c\u548c\u5168\u9762\u5206\u6790\u590d\u6742\u57fa\u56e0\u7ec4<\/strong>\u3002<\/p>\n\n\n\n\n\n\n

1. NovaSeq \u7684\u4e3b\u8981\u7279\u70b9<\/h3>\n\n\n\n

\u8d85\u9ad8\u541e\u5410\u91cf<\/h4>\n\n\n\n

NovaSeq \u6700\u91cd\u8981\u7684\u7279\u70b9\u662f\u6570\u636e\u541e\u5410\u91cf\u6781\u9ad8<\/strong>\uff1a\u5355\u6b21\u8fd0\u884c\u53ef\u751f\u6210\u9ad8\u8fbe 6 Tb<\/mark>\uff08\u592a\u6bd4\u7279\u6570\u636e\u5e93\uff09<\/strong>\u6216\u66f4\u591a\u7684\u6570\u636e\uff0c\u4f7f\u540c\u65f6\u5206\u6790\u6570\u767e\u4e2a<\/strong>\u5168\u4eba\u7c7b\u57fa\u56e0\u7ec4\u6210\u4e3a\u53ef\u80fd\u3002\u4e0e\u5176\u4ed6 Illumina \u6d4b\u5e8f\u4eea\u76f8\u6bd4\uff0c\u8fd9\u662f\u8fc4\u4eca\u4e3a\u6b62\u80fd\u751f\u6210\u6700\u591a\u6570\u636e\u7684\u6d4b\u5e8f\u4eea\u3002<\/p>\n\n\n\n

\u7075\u6d3b\u7684\u541e\u5410\u91cf\u9009\u9879<\/h4>\n\n\n\n

NovaSeq \u63d0\u4f9b\u4e00\u7cfb\u5217\u6d41\u52a8\u6c60\u9009\u9879<\/strong>\uff0c\u53ef\u6839\u636e\u9879\u76ee\u89c4\u6a21\u8c03\u6574\u6570\u636e\u541e\u5410\u91cf\u3002\u8fd9\u4f7f\u5f97\u4ece\u5c0f\u578b\u5230\u8d85\u5927\u578b\u7684\u6d4b\u5e8f\u9879\u76ee\u90fd<\/strong>\u80fd\u80dc\u4efb\u3002\u63d0\u4f9b\u56db\u79cd\u6d41\u52a8\u6c60\u7c7b\u578b:<\/p>\n\n\n\n

    \n
  1. S1 \u6d41\u52a8\u6c60<\/strong>\uff1a\u9002\u7528\u4e8e\u4e2d\u5c0f\u578b\u9879\u76ee\u3002\u6700\u591a\u53ef\u751f\u6210 770 Gb \u7684\u6570\u636e\u3002<\/li>\n\n\n\n
  2. S2 \u6d41\u52a8\u6c60<\/strong>\uff1a\u9002\u7528\u4e8e\u4e2d\u578b\u9879\u76ee\u3002\u53ef\u751f\u6210\u591a\u8fbe 1.6 Tb \u7684\u6570\u636e\u3002<\/li>\n\n\n\n
  3. S4 \u6d41\u52a8\u6c60<\/strong>\uff1a\u9002\u7528\u4e8e\u5927\u578b\u9879\u76ee\u3002\u53ef\u751f\u6210\u591a\u8fbe 6 Tb \u7684\u6570\u636e\u3002<\/li>\n\n\n\n
  4. SP \u6d41\u52a8\u6c60<\/strong>\uff1a\u541e\u5410\u91cf\u6700\u4f4e\u7684\u9009\u62e9\uff0c\u9002\u7528\u4e8e\u5177\u6709\u6210\u672c\u6548\u76ca\u7684\u9879\u76ee\u3002<\/li>\n<\/ol>\n\n\n\n

    \u9ad8\u901f\u6d4b\u5e8f<\/h4>\n\n\n\n

    NovaSeq \u80fd\u591f\u6781\u5feb\u5730\u751f\u6210\u6570\u636e\u3002\u5373\u4f7f\u4f7f\u7528\u6210\u5bf9\u672b\u7aef 150 \u78b1\u57fa\u5bf9<\/strong>\uff08150 bp \u00d7 2\uff09\u8bfb\u6570\uff0c\u4e5f\u80fd\u5728\u4e00\u5230\u4e24\u5929\u5185\u751f\u6210\u5927\u91cf\u6570\u636e\uff0c\u4ece\u800c\u4e3a\u8017\u65f6\u7684\u5168\u57fa\u56e0\u7ec4\u5206\u6790\u548c\u5927\u578b RNA \u6d4b\u5e8f<\/strong>\u9879\u76ee\u63d0\u4f9b\u5feb\u901f\u7ed3\u679c\u3002.<\/p>\n\n\n\n

    Illumina \u521b\u65b0\u6280\u672f\uff08SBS \u6280\u672f\u548c X \u901a\u9053\u5316\u5b66\u6280\u672f\uff09<\/h4>\n\n\n\n
      \n
    • \u5408\u6210\u6d4b\u5e8f\uff08SBS\uff09\u6280\u672f\uff1a<\/strong>
      NovaSeq \u548c Illumina \u7684\u5176\u4ed6\u6d4b\u5e8f\u4eea\u4e00\u6837\uff0c\u90fd\u91c7\u7528\u4e86 SBS \u6280\u672f\u3002\u8be5\u6280\u672f\u901a\u8fc7\u8367\u5149\u6807\u8bb0\u7684\u6838\u82f7\u9178\u4e00\u4e2a\u78b1\u57fa\u4e00\u4e2a\u78b1\u57fa\u5730\u5438\u6536\uff0c\u5b9e\u65f6\u68c0\u6d4b\u548c\u786e\u5b9a DNA \u5e8f\u5217\u3002\u53ef\u4ee5\u751f\u6210\u9ad8\u7cbe\u5ea6\u3001\u9ad8\u901a\u91cf\u7684\u6570\u636e\u3002<\/li>\n\n\n\n
    • X \u901a\u9053\u5316\u5b66\u6280\u672f<\/strong>\uff1a
      NovaSeq \u4f7f\u7528 Illumina \u6700\u65b0\u7684X \u901a\u9053\u5316\u5b66\u6280\u672f<\/strong>\uff0c\u6bd4\u4f20\u7edf\u7684\u53cc\u901a\u9053\u5316\u5b66\u6280\u672f<\/strong>\u66f4\u5feb\u3001\u66f4\u9ad8\u6548\u3002\u8fd9\u9879\u6280\u672f\u5927\u5927\u63d0\u9ad8\u4e86\u6d4b\u5e8f\u901f\u5ea6\uff0c\u5e76\u80fd\u5728\u6781\u77ed\u7684\u65f6\u95f4\u5185\u751f\u6210\u5927\u91cf\u6570\u636e\u3002<\/li>\n<\/ul>\n\n\n\n

      \u53cc\u7d22\u5f15\u9009\u9879<\/h4>\n\n\n\n

      NovaSeq \u652f\u6301\u53cc\u7d22\u5f15<\/strong>\uff0c\u53ef\u5728\u4e00\u6b21\u8fd0\u884c\u4e2d\u540c\u65f6\u5bf9\u6570\u5343\u4e2a\u6837\u672c<\/strong>\u8fdb\u884c\u6d4b\u5e8f\u3002\u8fd9\u6837\u5c31\u80fd\u9ad8\u6548\u5904\u7406\u591a\u4e2a\u6837\u672c\uff0c\u8282\u7701\u5927\u91cf\u65f6\u95f4\u548c\u91d1\u94b1\u3002<\/p>\n\n\n\n\n\n\n

      2. NovaSeq \u7684\u5e94\u7528<\/h3>\n\n\n\n

      NovaSeq \u5e7f\u6cdb\u7528\u4e8e\u9700\u8981\u9ad8\u901a\u91cf\u7684\u8d85\u5927\u578b\u6d4b\u5e8f\u9879\u76ee\u548c\u5546\u4e1a\u6d4b\u5e8f\u670d\u52a1<\/strong>\u3002\u4e3b\u8981\u5e94\u7528\u5305\u62ec<\/p>\n\n\n\n

      2.1 \u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\uff08WGS\uff09<\/h4>\n\n\n\n

      NovaSeq \u662f\u4eba\u7c7b\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f<\/strong>\u7684\u6807\u51c6\u578b\u53f7\uff0c\u53ef\u5bf9\u5927\u91cf\u4eba\u7fa4\u8fdb\u884c\u5168\u57fa\u56e0\u7ec4\u5206\u6790\u3002\u5b83\u9002\u7528\u4e8e\u4e2a\u4f53\u57fa\u56e0\u7ec4\u5256\u6790\u548c\u4e0e\u75be\u75c5\u76f8\u5173\u7684\u5927\u89c4\u6a21\u57fa\u56e0\u7ec4\u53d8\u5f02\u5206\u6790\uff1b\u4e00\u6b21\u8fd0\u884c\u53ef\u8986\u76d6\u591a\u4e2a\u4e2a\u4f53\u7684\u5168\u57fa\u56e0\u7ec4\uff0c\u56e0\u6b64\u7279\u522b\u9002\u7528\u4e8e\u764c\u75c7\u7814\u7a76<\/strong>\u548c\u7fa4\u4f53\u57fa\u56e0\u7ec4\u5b66<\/strong>\u9879\u76ee\u3002<\/p>\n\n\n\n

      2.2 \u8f6c\u5f55\u7ec4\u5206\u6790\uff08RNA-seq\uff09<\/h4>\n\n\n\n

      NovaSeq \u8fd8\u662f\u4f7f\u7528RNA \u6d4b\u5e8f\uff08RNA-seq\uff09<\/strong>\u8fdb\u884c\u57fa\u56e0\u8868\u8fbe\u5206\u6790<\/strong>\u4e2d\u8f6c\u5f55\u7ec4\u5206\u6790\u7684\u7406\u60f3\u9009\u62e9\uff0c\u56e0\u4e3a\u5b83\u53ef\u4ee5\u5728\u77ed\u65f6\u95f4\u5185\u83b7\u5f97\u5927\u91cf\u6570\u636e\u96c6\u3002\u4e00\u6b21\u53ef\u5b8c\u6210\u6570\u5341\u5230\u6570\u767e\u4e2a\u6837\u672c\u7684 RNA \u56fe\u8c31\u5206\u6790\u3002<\/p>\n\n\n\n

      2.3 \u5916\u663e\u5b50\u7ec4\u6d4b\u5e8f<\/h4>\n\n\n\n

      \u5916\u663e\u5b50\u7ec4\u6d4b\u5e8f<\/strong>\u4ee5\u57fa\u56e0\u7f16\u7801\u533a\u4e3a\u76ee\u6807\uff0c\u7528\u4e8e\u5168\u9762\u5206\u6790\u4e0e\u75be\u75c5\u76f8\u5173\u7684\u9057\u4f20\u53d8\u5f02\uff1bNovaSeq \u53ef\u540c\u65f6\u5bf9\u6570\u5343\u4e2a\u6837\u672c\u8fdb\u884c\u5916\u663e\u5b50\u7ec4\u6d4b\u5e8f\uff0c\u56e0\u6b64\u9002\u7528\u4e8e\u5927\u89c4\u6a21\u9057\u4f20\u53d8\u5f02\u9879\u76ee\u3002<\/p>\n\n\n\n

      2.4 \u591a\u76ee\u6807\u6d4b\u5e8f<\/h4>\n\n\n\n

      NovaSeq\u8fd8<\/strong>\u53ef\u7528\u4e8e\u764c\u75c7\u57fa\u56e0\u9762\u677f\u548c\u9057\u4f20\u75be\u75c5\u5206\u6790\u9762\u677f<\/strong>\u7b49\u9776\u5411\u6d4b\u5e8f<\/strong>\uff0c\u4ee5\u68c0\u6d4b\u5404\u79cd\u57fa\u56e0\u53d8\u5f02\u3002\u7531\u4e8e\u5b83\u80fd\u9ad8\u6548\u5730\u5206\u6790\u591a\u91cd\u6837\u672c\uff0c\u56e0\u6b64\u6210\u672c\u6548\u76ca\u4e5f\u5f88\u9ad8\u3002<\/p>\n\n\n\n

      2.5 \u5927\u89c4\u6a21\u5143\u57fa\u56e0\u7ec4\u5206\u6790<\/h4>\n\n\n\n

      NovaSeq \u8fd8\u7528\u4e8e\u5143\u57fa\u56e0\u7ec4\u5b66<\/strong>\uff08\u73af\u5883 DNA \u5206\u6790\uff09\uff0c\u4ee5\u5206\u6790\u73af\u5883\u4e2d\u5fae\u751f\u7269\u7fa4\u843d\u7684\u591a\u6837\u6027\u3001\u76d1\u6d4b\u75c5\u539f\u4f53\u548c\u7814\u7a76\u8fdb\u5316\u3002<\/p>\n\n\n

      \n
      \"\u89aa\u5b50\u9451\u5b9a\u3000NIPPT\u3000DNA\u9451\u5b9a\u3000\u7cbe\u6db2\"<\/figure><\/div>\n\n\n\n\n\n

      3. NovaSeq \u7684\u4f18\u52bf<\/h3>\n\n\n\n
        \n
      1. \u541e\u5410\u91cf\u6781\u9ad8<\/strong>\uff1a\u6570\u636e\u751f\u6210\u80fd\u529b\u9ad8\u8fbe 6 Tb\uff0c\u53ef\u5feb\u901f\u5206\u6790\u5927\u578b\u9879\u76ee\u548c\u6570\u5343\u4e2a\u6837\u672c\u3002<\/li>\n\n\n\n
      2. \u5feb\u901f\u83b7\u53d6\u6570\u636e<\/strong>\uff1aNovaSeq \u7684\u901f\u5ea6\u6781\u5feb\uff0c\u53ef\u5728\u4e00\u4e24\u5929\u5185\u751f\u6210\u5927\u91cf\u6d4b\u5e8f\u6570\u636e\uff0c\u5373\u4f7f\u662f\u5927\u578b\u3001\u8017\u65f6\u7684\u5206\u6790\u9879\u76ee\u4e5f\u80fd\u5feb\u901f\u5f97\u51fa\u7ed3\u679c\u3002<\/li>\n\n\n\n
      3. \u63d0\u9ad8\u6210\u672c\u6548\u76ca<\/strong>\uff1a\u9ad8\u901a\u91cf\u548c\u5904\u7406\u5927\u91cf\u6837\u672c\u7684\u80fd\u529b\u964d\u4f4e\u4e86\u6bcf\u4e2a\u6837\u672c\u7684\u6210\u672c\uff0c\u4f7f\u5176\u5728\u5927\u89c4\u6a21\u7814\u7a76\u4e2d\u5177\u6709\u5f88\u9ad8\u7684\u6548\u7387\u3002<\/li>\n\n\n\n
      4. \u5e94\u7528\u5e7f\u6cdb<\/strong>\uff1a\u5168\u57fa\u56e0\u7ec4\u5206\u6790\u3001RNA \u6d4b\u5e8f\u3001\u5916\u663e\u5b50\u7ec4\u5206\u6790\u3001\u9776\u5411\u6d4b\u5e8f\u548c\u5143\u57fa\u56e0\u7ec4\u5206\u6790\u3002<\/li>\n<\/ol>\n\n\n\n\n\n\n

        \u6458\u8981<\/h3>\n\n\n\n

        NovaSeq<\/strong>\u662f Illumina \u6700\u5f3a\u5927\u7684\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u4eea\uff0c\u5177\u6709\u6781\u9ad8\u7684\u6570\u636e\u541e\u5410\u91cf\u548c\u7075\u6d3b\u7684\u6d41\u5f0f\u7ec6\u80de\u9009\u62e9\uff0c\u662f\u5927\u89c4\u6a21\u57fa\u56e0\u7ec4\u5206\u6790\u9879\u76ee<\/strong>\u7684\u7406\u60f3\u9009\u62e9\u3002\u5b83\u80fd\u540c\u65f6\u5206\u6790\u5927\u91cf\u6837\u672c\uff0c\u5e76\u5728\u77ed\u65f6\u95f4\u5185\u751f\u6210\u6d77\u91cf\u6570\u636e\uff0c\u5305\u62ec\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u3001\u5916\u663e\u5b50\u7ec4\u5206\u6790\u548c RNA \u6d4b\u5e8f\uff0c\u56e0\u6b64\u88ab\u9700\u8981\u9ad8\u901a\u91cf<\/strong>\u5206\u6790\u7684\u7814\u7a76\u673a\u6784\u548c\u5546\u4e1a\u6d4b\u5e8f\u670d\u52a1\u673a\u6784\u5e7f\u6cdb\u4f7f\u7528\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

        NovaSeq \u662f\u65b0\u4e00\u4ee3\u6d4b\u5e8f\u7684\u9886\u5148\u578b\u53f7\uff0c\u63d0\u4f9b\u591a\u79cd\u6570\u636e\u541e\u5410\u91cf\u9009\u9879\uff0c\u7528\u4e8e\u5206\u6790\u5927\u91cf\u6837\u672c\u548c\u5168\u9762\u5206\u6790\u590d\u6742\u57fa\u56e0\u7ec4\u3002 1. NovaSeq \u7684\u4e3b\u8981\u7279\u70b9 \u8d85\u9ad8\u541e\u5410\u91cf NovaSeq \u6700\u91cd…<\/p>\n","protected":false},"author":5,"featured_media":3146,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[31],"tags":[81,78,40,66,83,67,68],"class_list":{"0":"post-6208","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-genetic-testing-zh-hans","8":"tag-dna_profiling-zh-hans","9":"tag-dna_test-zh-hans","10":"tag-expert-zh-hans","11":"tag-accuracy-zh-hans","12":"tag-result-zh-hans","13":"tag-genetic_testing-zh-hans","14":"tag-analysis-zh-hans","15":"article"},"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6208","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/comments?post=6208"}],"version-history":[{"count":2,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6208\/revisions"}],"predecessor-version":[{"id":6221,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/posts\/6208\/revisions\/6221"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media\/3146"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/media?parent=6208"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/categories?post=6208"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nippt\/wp-json\/wp\/v2\/tags?post=6208"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}