{"id":111494,"date":"2024-06-30T10:32:33","date_gmt":"2024-06-30T01:32:33","guid":{"rendered":"https:\/\/www.hiro-clinic.or.jp\/nipt\/about-forenseq-mainstay-kit\/"},"modified":"2025-08-21T18:25:56","modified_gmt":"2025-08-21T09:25:56","slug":"about-forenseq-mainstay-kit","status":"publish","type":"post","link":"https:\/\/www.hiro-clinic.or.jp\/nipt\/about-forenseq-mainstay-kit\/?lang=en","title":{"rendered":"About the ForenSeq MainstAY Kit"},"content":{"rendered":"\n<div style=\"border:solid #ffe6e6 0.8rem;background-color: #fff9f9;padding:3% 5%;margin:1rem 0 3rem;\">\n<h2 style=\"margin-top:1rem;\">\u3053\u306e\u8a18\u4e8b\u306e\u307e\u3068\u3081<\/h2>\n<p>The ForenSeq MainstAY Kit is a next-generation sequencing (NGS)-based kit developed by Verogen, a subsidiary of Illumina, specifically designed for forensic DNA profiling. This kit enables highly accurate and efficient DNA analysis in the field of forensics.<\/p><\/div>\n\n\n\n\n\n\n<div id=\"ez-toc-container\" class=\"ez-toc-v2_0_84 counter-hierarchy ez-toc-counter ez-toc-grey ez-toc-container-direction\">\n<div class=\"ez-toc-title-container\">\n<p class=\"ez-toc-title\" style=\"cursor:inherit\">\u76ee\u6b21<\/p>\n<span class=\"ez-toc-title-toggle\"><a href=\"#\" class=\"ez-toc-pull-right ez-toc-btn ez-toc-btn-xs ez-toc-btn-default ez-toc-toggle\" aria-label=\"Toggle Table of Content\"><span class=\"ez-toc-js-icon-con\"><span class=\"\"><span class=\"eztoc-hide\" style=\"display:none;\">Toggle<\/span><span class=\"ez-toc-icon-toggle-span\"><svg style=\"fill: #999;color:#999\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" class=\"list-377408\" width=\"20px\" height=\"20px\" viewBox=\"0 0 24 24\" fill=\"none\"><path d=\"M6 6H4v2h2V6zm14 0H8v2h12V6zM4 11h2v2H4v-2zm16 0H8v2h12v-2zM4 16h2v2H4v-2zm16 0H8v2h12v-2z\" fill=\"currentColor\"><\/path><\/svg><svg style=\"fill: #999;color:#999\" class=\"arrow-unsorted-368013\" xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"10px\" height=\"10px\" viewBox=\"0 0 24 24\" version=\"1.2\" baseProfile=\"tiny\"><path d=\"M18.2 9.3l-6.2-6.3-6.2 6.3c-.2.2-.3.4-.3.7s.1.5.3.7c.2.2.4.3.7.3h11c.3 0 .5-.1.7-.3.2-.2.3-.5.3-.7s-.1-.5-.3-.7zM5.8 14.7l6.2 6.3 6.2-6.3c.2-.2.3-.5.3-.7s-.1-.5-.3-.7c-.2-.2-.4-.3-.7-.3h-11c-.3 0-.5.1-.7.3-.2.2-.3.5-.3.7s.1.5.3.7z\"\/><\/svg><\/span><\/span><\/span><\/a><\/span><\/div>\n<nav><ul class='ez-toc-list ez-toc-list-level-1 ' ><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-1\" href=\"https:\/\/www.hiro-clinic.or.jp\/nipt\/about-forenseq-mainstay-kit\/?lang=en\/#Key_Features_and_Applications\" >Key Features and Applications<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-2\" href=\"https:\/\/www.hiro-clinic.or.jp\/nipt\/about-forenseq-mainstay-kit\/?lang=en\/#Kit_Composition_and_Operation\" >Kit Composition and Operation<\/a><\/li><li class='ez-toc-page-1 ez-toc-heading-level-2'><a class=\"ez-toc-link ez-toc-heading-3\" href=\"https:\/\/www.hiro-clinic.or.jp\/nipt\/about-forenseq-mainstay-kit\/?lang=en\/#Summary\" >Summary<\/a><\/li><\/ul><\/nav><\/div>\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Key_Features_and_Applications\"><\/span><strong>Key Features and Applications<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Targeted sequencing<\/strong><strong><br><\/strong> The ForenSeq MainstAY Kit simultaneously analyzes <strong>52 forensically relevant genetic markers<\/strong>, including <strong>27 autosomal STRs<\/strong> and <strong>25 Y-STRs<\/strong>. This enables high-precision DNA profiling for criminal investigations and paternity testing.<br><\/li>\n\n\n\n<li><strong>High accuracy and reproducibility<\/strong><strong><br><\/strong> Validation data demonstrate exceptionally high accuracy \u2014 <strong>99.82% for autosomal STRs<\/strong> and <strong>100% for Y-STRs<\/strong> \u2014 along with consistent reproducibility, ensuring highly reliable results.<br><\/li>\n\n\n\n<li><strong>Broad range of applications<\/strong><strong><br><\/strong> This kit is suitable for a wide array of forensic purposes, such as analyzing crime scene samples, identifying remains, and verifying familial relationships. It is also compatible with major international databases like <strong>CODIS<\/strong> and the <strong>European Standard Set (ESS)<\/strong>.<br><\/li>\n\n\n\n<li><strong>Rapid data analysis<\/strong><strong><br><\/strong> When paired with the <strong>MiSeq FGx Sequencing System<\/strong>, it enables efficient data analysis and automated report generation, significantly improving the turnaround time for forensic laboratories.<br><\/li>\n<\/ul>\n\n\n\n<p><strong>Cost-effective solution<\/strong><strong><br><\/strong> Compared to traditional <strong>capillary electrophoresis (CE)<\/strong> methods, this kit offers greater cost efficiency and utilizes <strong>NGS technology<\/strong> to provide more comprehensive and detailed DNA information.<\/p>\n\n\n\n    <a href=\"\/nipt\/difference-between-ccfdna-tube-and-dna-bct\/\" class=\"blog-card\">\n      <div class=\"blog-card-content\">\n          <div class=\"blog-card-title\">PAXgene Blood ccfDNA Tube\u3068Streck Cell-Free DNA BCT\u306e\u9055\u3044 <\/div>\n          <div class=\"blog-card-excerpt\">...<\/div>\n      <\/div>\n    <\/a>\n\n\n\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" width=\"640\" height=\"427\" src=\"\/nipt\/wp-content\/uploads\/2024\/12\/29217899_s.jpg\" alt=\"\u907a\u4f1d\u5b50\" class=\"wp-image-86530\"\/><\/figure><\/div>\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Kit_Composition_and_Operation\"><\/span><strong>Kit Composition and Operation<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Recommended DNA input<\/strong>: <strong>1 ng of genomic DNA (gDNA)<\/strong> per sample.<br><\/li>\n\n\n\n<li><strong>Reaction formats<\/strong>: Available in <strong>96-reaction<\/strong> and <strong>384-reaction<\/strong> formats, ideal for high-throughput sample processing.<br><\/li>\n<\/ul>\n\n\n\n<p><strong>Library preparation time<\/strong>: Approximately <strong>7 hours and 15 minutes<\/strong> in total, with just <strong>1 hour and 30 minutes<\/strong> of hands-on time for efficient workflow.<\/p>\n\n\n\n    <a href=\"\/nipt\/about-the-difference-in-mechanism\/\" class=\"blog-card\">\n      <div class=\"blog-card-content\">\n          <div class=\"blog-card-title\">QIAGEN ForenSeq MainstAY Kit\u3068ForenSeq DNA Signature Prep Kit\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u9055\u3044\u306b\u3064\u3044\u3066 <\/div>\n          <div class=\"blog-card-excerpt\">...<\/div>\n      <\/div>\n    <\/a>\n\n\n\n\n\n\n<h2 class=\"wp-block-heading\"><span class=\"ez-toc-section\" id=\"Summary\"><\/span><strong>Summary<\/strong><span class=\"ez-toc-section-end\"><\/span><\/h2>\n\n\n\n<p>\u00a0The ForenSeq MainstAY Kit is a <strong>powerful tool for forensic DNA profiling<\/strong>, delivering high-precision and reliable results for criminal investigations, paternity testing, and human identification. By leveraging <strong>NGS technology<\/strong>, it enables rapid and efficient generation of detailed DNA information, making it an essential asset for modern forensic laboratories. For more information, refer to the official resources from <strong>Illumina<\/strong> or <strong>Verogen<\/strong>.<\/p>\n","protected":false},"excerpt":{"rendered":"\u3053\u306e\u8a18\u4e8b\u306e\u307e\u3068\u3081 The ForenSe&#8230;\n <a href=\"https:\/\/www.hiro-clinic.or.jp\/nipt\/about-forenseq-mainstay-kit\/?lang=en\">\u7d9a\u304d\u3092\u8aad\u3080<\/a>","protected":false},"author":59,"featured_media":79929,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[97],"tags":[],"class_list":["post-111494","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/posts\/111494","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/users\/59"}],"replies":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/comments?post=111494"}],"version-history":[{"count":2,"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/posts\/111494\/revisions"}],"predecessor-version":[{"id":111500,"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/posts\/111494\/revisions\/111500"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/media\/79929"}],"wp:attachment":[{"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/media?parent=111494"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/categories?post=111494"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.hiro-clinic.or.jp\/nipt\/wp-json\/wp\/v2\/tags?post=111494"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}