What kind of next-generation sequencer is MiniSeq?

Main features of MiniSeq

Compact design:

• MiniSeq is much smaller than Illumina’s other sequencers and is designed to be easily installed in limited space in the laboratory. This provides an environment where next-generation sequencing technology can be easily used.

Easy operation:

• MiniSeq is designed to be easy to handle for the NGS novice with intuitive software and automated protocols. The integrated workflow from sample preparation to data analysis makes it simple and fast to obtain results.

Ideal for targeted sequencing:

• MiniSeq is ideal for targeted region sequencing (sequencing to analyze a specific gene or genomic region). It is widely used in studies such as analysis of cancer-related genes, genetic testing for hereditary diseases, and identification of microorganisms.

Cost-effective sequence:

• Optimized for small-scale projects, it can be used in laboratories with limited budgets because of its lower initial investment and running costs compared to large-scale next-generation sequencers.

Rapid data acquisition:

• The MiniSeq provides rapid sequencing for small samples. In a typical experiment, samples are often analyzed in less than a day, making it ideal for studies that require rapid results.

MiniSeq Technology

MiniSeq uses a reversible terminator-based sequencing technology (SBS: Sequencing by Synthesis) developed by Illumina. This technology has the following features

Sequencing Bisynthesis (SBS) technology:

• SBS is a method of reading nucleotide sequences one base at a time using a synthetic reaction of DNA. As fluorescently labeled nucleotides are incorporated according to the DNA template, each base is detected sequentially and the DNA sequence is determined.

Read length and data throughput:

• The MiniSeq can adjust the length of sequencing reads (length of sequence to be read) from 100 base pairs (bp) to as low as 150 bp. In addition, data throughput is designed for small projects and can generate data volumes up to 7.5 Gb.

MiniSeq Applications

The MiniSeq is used in a variety of research fields. The main applications are as follows

Target sequencing:

• It is used for sequencing of specific genes or genomic regions. It is used to test for cancer-related genes, genetic diseases, and to identify microorganisms.

Gene expression analysis:

• MiniSeq also supports RNA sequencing, which is used to measure gene expression levels. This allows analysis of gene expression patterns under specific conditions to investigate disease and therapeutic response.

Microbial genome analysis:

• It is used for genome analysis of microorganisms and identification of pathogens, and has been applied to research on infectious diseases and antibiotic resistance.

Cancer Research:

• MiniSeq is used to analyze genetic mutations and tumor-related biomarkers in cancer. Targeted sequencing allows rapid identification of specific genetic mutations in cancer cells.

summary:

MiniSeq is a compact, easy-to-use next-generation sequencer from Illumina designed primarily for small-scale projects such as targeted sequencing and gene expression analysis. It offers rapid data acquisition and cost-effective operation, and is suitable for a wide range of laboratory applications.

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Supervisor of the article

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Dr. Hiroshi Oka

Director of CAP Laboratory

Graduated from Keio University, Faculty of Medicine

Doctor of Medicine

Medical Doctor