The person’s DNA can be extracted relatively easily from saliva.Saliva contains cells from the oral cavity (mainly mucosal cells from the inner cheek) and white blood cells from which DNA can be extracted.Saliva-based DNA collection is a non-invasive and simple method and is widely used for the following reasons

1. The convenience of DNA extraction from saliva

Non-invasive method: saliva can be easily collected without the needles and needles like blood.This is commonly used for DNA and genetic testing as it is painless and easy to carry out without pain or strain.
Can be collected at home: saliva samples can be collected at home using swabs or special containers. This is a convenient and accessible method, as the sample can be collected and mailed to a laboratory for DNA analysis.

2. The procedure for DNA extraction from saliva

The procedure for DNA extraction using saliva is as follows

Sample collection: spit saliva directly into a container or collect mucosa cells from the inner cheek with a cotton swab.Care should be taken not to mix food debris or foreign bodies in this process.
Extraction of DNA: DNA is extracted from cells in saliva. This is typically done using DNA extraction kits or enzyme-based methods. The extracted DNA is amplified to a quantity suitable for analysis.
DNA analysis: extracted DNA is used for genotyping and personal identification.

3.The advantages of DNA extraction from saliva

Simple and efficient: DNA extraction from saliva is easier than blood or semen and is easier to collect and process. It contains sufficient amounts of DNA for general testing and genetic analysis, thus providing reliable results.
Easy storage: saliva samples can be stored for relatively long periods of time if stored properly.The use of special preservation solutions can also prevent sample deterioration and maintain DNA quality.

4. Applications of salivary DNA extraction

DNA extracted from saliva is used to

Paternity and genetic testing: DNA is extracted from saliva samples to confirm parent-child relationships and to analyse genetic characteristics.
Medical and genetic testing: saliva is also used for testing for genetic mutations and for genetic testing to analyse individual constitution and disease risk.

5. Precautions in DNA extraction from saliva .

Beware of contaminants: it is recommended to avoid consuming food or drinks immediately prior to saliva collection to avoid contamination with food debris or DNA from other people.This will ensure sample quality.
Ensuring quantity: it is important to collect sufficient quantities of saliva. In particular, a firm collection from the cell-rich areas of the oral mucosa will provide higher quality DNA.

Summary

Extracting DNA from saliva is relatively simple and is widely used as a non-invasive and efficient method. It is used for paternity testing, genetic testing and personal identification, and samples can easily be collected and sent for testing at home. Saliva is easy to store and contains sufficient amounts of DNA to provide reliable results.

医師監修監修日：2024年9月17日

岡博史（おかひろし）

医師・医学博士／ヒロクリニック統括院長

YouTubeチャンネルなどを通じて、遺伝子やNIPT（新型出生前診断）についてわかりやすく発信しています。

略歴

1996年慶應義塾大学医学部卒業
2004年慶應義塾大学医学博士号取得
2005年慶應義塾大学皮膚科学教室助手
2008年ヒロ皮フ形成クリニック開業
2009年医療法人社団福美会理事長
2015年医療法人社団福美会理事

資格・所属

CAPラボディレクター
日本皮膚科学会皮膚科専門医
日本医師会産業医
東京衛生検査所指導監督医

発信・関連リンク

この記事は、ヒロクリニックNIPPTの編集・監修体制にもとづき、資格を持つ医師が内容を確認しています。

医師監修監修日：2024年9月17日

堤修一（つつみしゅういち）

医師・医学博士／ヒロクリニック博多駅前院院長

略歴

1993年東京大学医学部医学科卒業
2016〜2019年東京大学先端科学技術研究センター准教授

発信・関連リンク

プロフィール詳細

この記事は、ヒロクリニックNIPPTの編集・監修体制にもとづき、資格を持つ医師が内容を確認しています。

参考文献

Abraham JE, Maranian MJ, Spiteri I, et al. Saliva samples as a source of DNA for high throughput genotyping. BMC Res Notes. 2012;5:137.
Rogers NL, Cole SA, Lan HC, et al. New saliva DNA collection method compared to buccal swabs in the detection of single nucleotide polymorphisms. Am J Hum Biol. 2007;19(3):319-26.