Amniotic Fluid Test

NIPT (non-invasive prenatal testing) is not a definitive test. It is possible for a positive diagnosis to be a ‘false positive’, meaning that the baby is negative but is diagnosed as positive for a variety of reasons. For this reason, we recommend that those who test positive have an amniotic fluid test as a definitive test.

Hiro Clinic NIPT offers a mutual aid society sponsored by affiliated laboratories.
Patients with positive test results are eligible for up to 200,000 yen in subsidies for the cost of amniotic fluid testing.
We have established criteria for amniotic fluid testing cost assistance. Please refer to Mutual Aid Society for details.

In addition, Second Tokyo Clinical Laboratory, with which Hiro Clinic NIPT is affiliated, offers amniotic fluid genetic testing for those who have been tested at affiliated facilities including Hiro Clinic NIPT and tested positive. The cost of the amniotic fluid test will be covered by Hiro Clinic NIPT.

For those who wish to have an amniotic fluid test >>

Amniotic fluid testing at Second Tokyo Clinical Laboratory

Second Tokyo Clinical Laboratory offers real-time PCR testing for amniotic fluid. This is a PCR test for trisomies 21, 18, and 13 in amniotic fluid.

What is an amniotic fluid test? >>

Real-Time PCR Test

Real-time PCR testing of amniotic fluid is determined by exponentially increasing the number of target gene sequence molecules and measuring the amount of genes.

What is the advantage?

The G-band method, which is often used in amniotic fluid testing, requires cell culture, which takes time.
The advantage of the real-time PCR test is that it does not require cell culture and provides results in a relatively short period of time.

What does it test for?

Abnormalities in the number of chromosomes (trisomy, monosomy, etc.)

  • Detection of the presence or absence of abnormal number of chromosomes (trisomy, monosomy, etc.)

What is an amniotic fluid test?

Common amniotic fluid tests conventionally performed include specific genetic analysis methods such as the chromosome banding method and FISH method, as explained on Hiro Clinic NIPT page. These tests can reveal the presence or absence of relatively rough genetic abnormalities such as abnormal chromosome numbers or structural abnormalities (inversions, translocations, large deletions or duplications), such as the trisomy 21 trisomy that causes Down syndrome.

However, it is difficult to comprehensively detect microscopic structural abnormalities in genes with conventional testing methods, and numerous genetic abnormalities are likely to be missed. Therefore, a new era is coming in which comprehensive analysis by exome analysis and whole genome analysis using next-generation sequencers that can detect DNA sequence abnormalities in a wide range of regions on a one-by-one basis will be highly demanded.

As for the collection of amniotic fluid samples, it is possible to analyze the genes of fetal-derived cells from a small amount of amniotic fluid in the same way as with the conventional method. Compared to the conventional method, there is no significant burden on the patient.

Do you want to undergo Amniotic Fluid Testing?

After consulting with your obstetrician about the amniotic fluid test and once you have made a request, please send your amniotic fluid to Second Tokyo Clinical Laboratory. If you are denied for the test, we can provide you with information on facilities that perform the test.
Do not hesitate to contact us for more information.

How to collect Amniotic Fluid?

  1. Collect 15 ml of amniotic fluid in a 15 ml sterile tube provided by the amniotic fluid collection medical facility.
  2. Place it in a 50 ml tube and wrap the screw cap with Parafilm.
  3. Place in biohazardous vinyl with zipper and wrap with absorbent sheet.
  4. Please keep refrigerated until it is collected.

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