B: over35 Plan

¥150,000  ( ¥165,000 including tax)  
¥192,500for twins, including tax
Via Partner Clinic
(¥187,000 including tax)
¥214,500 for twins, including tax

The over35 plan examines for Patau Syndrome, Edwards Syndrome, and Down Syndrome which are among the most frequent congenital diseases caused by changes in the number of autosomal chromosomes, which are 22 pairs of chromosomes (44 chromosomes). This plan will also examine for trisomy and monosomy of all chromosomes 1-22 and sex chromosomes.

Determination of gender is possible for single fetus.

  • ● Tests abnormalities in Chromosomes 1-22.

  • ● For single fetus, abnormalities of sex chromosomes and gender/sex will be reported.

    Others …
  • ● For twins, you will be informed whether or not a Y chromosome is present.


Diseases that can be detected by this test

ChromosomeDisease/DisorderOccurrence rate
21Down syndrome1 in 700
18Edwards syndrome1 in 6,000
13Patau syndrome1 in 10,000
1Trisomy/Monosomy 1
2Trisomy/Monosomy 2
3Trisomy/Monosomy 3
4Trisomy/Monosomy 4
5Trisomy/Monosomy 5
6Trisomy/Monosomy 6
7Trisomy/Monosomy 7
8Trisomy/Monosomy 8
ChromosomeDisease/DisorderOccurrence rate
9Trisomy/Monosomy 9
10Trisomy/Monosomy 10
11Trisomy/Monosomy 11
12Trisomy/Monosomy 12
14Trisomy/Monosomy 14
15Trisomy/Monosomy 15
16Trisomy/Monosomy 16
17Trisomy/Monosomy 17
19Trisomy/Monosomy 19
20Trisomy/Monosomy 20
22Trisomy/Monosomy 22
ChromosomeDisease/DisorderOccurrence rate
XKlinefelter’s syndrome1 in 1,000
XTurner’s syndrome1 in 2,500
XXXX syndrome1 in 1,000
YXYY syndrome1 in 1,000

X-linked Recessive Inherited Disorder

The following is a list of the major X-linked recessive inherited diseases.

Boys may develop the disease when they have a close relative with X-linked recessive inherited disease, while girls are often asymptomatic carriers.
Note that NIPT cannot determine the presence or absence of X-linked recessive inherited disease. However, it is possible to verify the possibility of developing X-linked recessive inherited disease in a relative by examining the sex of the child.
The following are typical X-linked recessive genetic disorders:

ChromosomeDisease/DisorderOccurrence rate
XX-linked agammaglobulinemia (XLA)Boys 1 in 100,000
XGlucose-6-phosphate dehydrogenase deficiency1 in 1,000
XX-linked hypophosphatemic rickets (XLH)1 in 20,000
XRed-green colorblindness1 in 20 to 500
XHemophilia1 in 4 boys with maternal retention
ChromosomeDisease/DisorderOccurrence rate
XDuchenne muscular dystrophy (DMD)60% for boys 1 in 3,500 maternal retention
XX-linked ichthyosis1 in 2,000 to 6,000
XKallmann syndrome1 in 10,000
X, YLeri-Weill dyschondrosteosis (LWD) 

Recommended to:

Those who are over 35 years of age. Since trisomy of all chromosomes tends to occur more frequently in this age group.

A quarter of those with positive results didn’t know they turned positive

2.45%( around 518 people) of the total number of patients in the Hiro Clinic NIPT had positive results. (*As of March 2020 – June 2021)
About 27.3% of them were not able to find out that they had tested positive for a disease other than the ones that are reported/tested by the plan they selected. This includes 7.31% of those who chose the over35 plan.

Positivity Report

Hiro Clinic Hiro Clinic Positivity Report Positivity Report

Source: Hiro Clinic Research

Assuming 100% of those who were positive, whether or not the results are reported.

The 17.4% of all the pregnant women who took the over35 plan were detected to have chromosomal abnormalities not included in to the test coverage: Chromosome 1~22 trisomy/monosomy and sex chromosome abnormality.

Below is a chart showing the range of diseases that can be reported under the over35 Plan based on the percentage of overall disease types for those who are found to have a disease/disorder.

Scope of report on results of Full Set & over35 Plan

Hiro Clinic Hiro Clinic NIPT NIPT over35 Plan over35 Plan

Source: Hiro Clinic Research

Consider a Full set plan that includes a very detailed examination.

The over35 plan can examine one fetus (single fetus) or twins.
All clinic tests are performed domestically in Japan, and results are usually delivered within 2~5 days (usually within 3~6 days for some clinics and partner facilities) from the day of blood collection. Please note that express delivery option is available at all Hiro Clinic NIPT clinics (within 2~3 days after blood collection), except for partner facilities.