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この記事のまとめ
The ForenSeq MainstAY Kit is a next-generation sequencing (NGS)-based kit developed by Verogen, a subsidiary of Illumina, specifically designed for forensic DNA profiling. This kit enables highly accurate and efficient DNA analysis in the field of forensics.
Key Features and Applications
- Targeted sequencing
The ForenSeq MainstAY Kit simultaneously analyzes 52 forensically relevant genetic markers, including 27 autosomal STRs and 25 Y-STRs. This enables high-precision DNA profiling for criminal investigations and paternity testing. - High accuracy and reproducibility
Validation data demonstrate exceptionally high accuracy — 99.82% for autosomal STRs and 100% for Y-STRs — along with consistent reproducibility, ensuring highly reliable results. - Broad range of applications
This kit is suitable for a wide array of forensic purposes, such as analyzing crime scene samples, identifying remains, and verifying familial relationships. It is also compatible with major international databases like CODIS and the European Standard Set (ESS). - Rapid data analysis
When paired with the MiSeq FGx Sequencing System, it enables efficient data analysis and automated report generation, significantly improving the turnaround time for forensic laboratories.
Cost-effective solution
Compared to traditional capillary electrophoresis (CE) methods, this kit offers greater cost efficiency and utilizes NGS technology to provide more comprehensive and detailed DNA information.
Kit Composition and Operation
- Recommended DNA input: 1 ng of genomic DNA (gDNA) per sample.
- Reaction formats: Available in 96-reaction and 384-reaction formats, ideal for high-throughput sample processing.
Library preparation time: Approximately 7 hours and 15 minutes in total, with just 1 hour and 30 minutes of hands-on time for efficient workflow.
Summary
The ForenSeq MainstAY Kit is a powerful tool for forensic DNA profiling, delivering high-precision and reliable results for criminal investigations, paternity testing, and human identification. By leveraging NGS technology, it enables rapid and efficient generation of detailed DNA information, making it an essential asset for modern forensic laboratories. For more information, refer to the official resources from Illumina or Verogen.
